The ontogeny of the endocrine pancreas in the fetal/newborn baboon

Amy R. Quinn, Cynthia L. Blanco, Carla Perego, Giovanna Finzi, Stefano La Rosa, Carlo Capella, Rodolfo Guardado-Mendoza, Francesca Casiraghi, Amalia Gastaldelli, Marney Johnson, Edward J. Dick, Franco Folli

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Erratic regulation of glucose metabolism including hyperglycemia is a common condition in premature infants and is associated with increased morbidity and mortality. The objective of this study was to examine histological and ultrastructural differences in the endocrine pancreas in fetal (throughout gestation) and neonatal baboons. Twelve fetal baboons were delivered at 125 days (d) gestational age (GA), 140d GA, or 175d GA. Eight animals were delivered at term (185d GA); half were fed for 5 days. Seventy-three nondiabetic adult baboons were used for comparison. Pancreatic tissue was studied using light microscopy, confocal imaging, and electron microscopy. The fetal and neonatal endocrine pancreas islet architecture became more organized as GA advanced. The percent areas of α-β-δ-cell type were similar within each fetal and newborn GA (NS) but were higher than the adults (P<0.05) regardless of GA. The ratio of β cells within the islet (whole and core) increased with gestation (P<0.01). Neonatal baboons, which survived for 5 days (feeding), had a 2.5-fold increase in pancreas weight compared with their counterparts killed at birth (PZ0.01). Endocrine cells were also found in exocrine ductal and acinar cells in 125, 140 and 175dGAfetuses. Subpopulation of tissue that coexpressed trypsin and glucagon/insulin shows the presence of cellswithmixed endo-exocrine lineage in fetuses. In summary, the fetal endocrine pancreas has no prevalence of a α-β-δ-cell type with larger endocrine cell percent areas than adults. Cells with mixed endocrine/exocrine phenotype occur during fetal development. Developmental differences may play a role in glucose homeostasis during the neonatal period and may have long-term implications.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalJournal of Endocrinology
Volume214
Issue number3
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Quinn, A. R., Blanco, C. L., Perego, C., Finzi, G., La Rosa, S., Capella, C., Guardado-Mendoza, R., Casiraghi, F., Gastaldelli, A., Johnson, M., Dick, E. J., & Folli, F. (2012). The ontogeny of the endocrine pancreas in the fetal/newborn baboon. Journal of Endocrinology, 214(3), 289-299. https://doi.org/10.1530/JOE-12-0070