The novel σ54- and σ28-dependent flagellar gene transcription hierarchy of Vibrio cholerae

M. G. Prouty, N. E. Correa, K. E. Klose

Research output: Contribution to journalArticle

149 Scopus citations

Abstract

The human pathogen Vibrio cholerae is a highly motile organism by virtue of a polar flagellum. Flagellar transcriptional regulatory factors have been demonstrated to contribute to V. cholerae virulence, but the role these factors play in the transcription hierarchy controlling flagellar synthesis has been unclear. The flagellar genes revealed by the V. cholerae genome sequence are located in three large clusters, with the exception of the motor genes, which are found in three additional locations. It had previously been demonstrated that the alternative sigma factor σ54 and the σ54-dependent activators FlrA and FlrC are necessary for flagellar synthesis. The V. cholerae genome sequence revealed the presence of a fliA gene, which is predicted to encode the alternative flagellar sigma factor σ28. A V. cholerae ΔfliA mutant strain is non-motile, and synthesizes a truncated flagellum. Vibrio cholerae FliA complements both V. cholerae and Salmonella typhimurium fliA mutants for motility, consistent with its function as an alternative flagellar sigma factor. Analysis of lacZ transcriptional fusions of the V. cholerae flagellar promoters in both V. cholerae and S. typhimurium identified σ28-, σ54, FlrA- and FlrC-dependent promoters, as well as promoters that were independent of all these factors. Our results support a model of V. cholerae flagellar gene transcription as a novel hierarchy composed of four classes of genes. Class I is composed solely of the gene encoding the σ54-dependent activator FlrA, which along with the σ54-holoenzyme form of RNA polymerase activates expression of Class II genes. These genes include structural components of the MS ring, switch and export apparatus, as well as the genes encoding both FliA and FlrC. FlrC, along with σ54-holoenzyme, activates expression of Class III genes, which include basal body, hook and filament genes. Finally, σ28. holoenzyme activates expression of Class IV genes, which include additional filament genes as well as motor genes. Thus, this novel V. cholerae flagellar hierarchy has incorporated elements from both the σ54-dependent Caulobacter crescentus polar flagellar hierarchy and the σ28-dependent S. typhimurium peritrichous flagellar hierarchy.

Original languageEnglish (US)
Pages (from-to)1595-1609
Number of pages15
JournalMolecular Microbiology
Volume39
Issue number6
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The novel σ<sup>54</sup>- and σ<sup>28</sup>-dependent flagellar gene transcription hierarchy of Vibrio cholerae'. Together they form a unique fingerprint.

  • Cite this