The non-human primate kidney transcriptome in fetal development

Kimberly D. Spradling-Reeves; Jeremy P. Glenn; Kenneth J. Lange; Natalia Kuhn; Jacqueline J Coalson; Mark J. Nijland; Cun Li; Peter W. Nathanielsz; Laura Cox

doi:10.1111/jmp.12340

The non-human primate kidney transcriptome in fetal development

Kimberly D. Spradling-Reeves, Jeremy P. Glenn, Kenneth J. Lange, Natalia Kuhn, Jacqueline J Coalson, Mark J. Nijland, Cun Li, Peter W. Nathanielsz, Laura Cox

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Background: Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods: The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results: Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions: This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.

Original language	English (US)
Pages (from-to)	157-171
Number of pages	15
Journal	Journal of medical primatology
Volume	47
Issue number	3
DOIs	https://doi.org/10.1111/jmp.12340
State	Published - Jun 2018

Keywords

array
baboon
cluster analysis
gene expression
ontogeny
pathway analysis

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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10.1111/jmp.12340

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title = "The non-human primate kidney transcriptome in fetal development",

abstract = "Background: Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods: The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results: Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions: This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.",

keywords = "array, baboon, cluster analysis, gene expression, ontogeny, pathway analysis",

author = "Spradling-Reeves, {Kimberly D.} and Glenn, {Jeremy P.} and Lange, {Kenneth J.} and Natalia Kuhn and Coalson, {Jacqueline J} and Nijland, {Mark J.} and Cun Li and Nathanielsz, {Peter W.} and Laura Cox",

note = "Funding Information: The authors acknowledge grant support from NIH P01 HD021350 and facilities support from NIH Research Facilities Improvement Program Grants C06 RR013556 and C06 RR017515. Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2018",

month = jun,

doi = "10.1111/jmp.12340",

language = "English (US)",

volume = "47",

pages = "157--171",

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AU - Spradling-Reeves, Kimberly D.

AU - Glenn, Jeremy P.

AU - Lange, Kenneth J.

AU - Kuhn, Natalia

AU - Coalson, Jacqueline J

AU - Nijland, Mark J.

AU - Li, Cun

AU - Nathanielsz, Peter W.

AU - Cox, Laura

N1 - Funding Information: The authors acknowledge grant support from NIH P01 HD021350 and facilities support from NIH Research Facilities Improvement Program Grants C06 RR013556 and C06 RR017515. Publisher Copyright: © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2018/6

Y1 - 2018/6

N2 - Background: Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods: The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results: Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions: This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.

AB - Background: Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods: The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results: Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions: This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.

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KW - pathway analysis

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The non-human primate kidney transcriptome in fetal development

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ASJC Scopus subject areas

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