The non-human primate kidney transcriptome in fetal development

Kimberly D. Spradling-Reeves, Jeremy P. Glenn, Kenneth J. Lange, Natalia Kuhn, Jacqueline J. Coalson, Mark J. Nijland, Cun Li, Peter W. Nathanielsz, Laura A. Cox

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background: Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods: The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results: Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions: This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.

Original languageEnglish (US)
Pages (from-to)157-171
Number of pages15
JournalJournal of medical primatology
Volume47
Issue number3
DOIs
StatePublished - Jun 2018

Keywords

  • array
  • baboon
  • cluster analysis
  • gene expression
  • ontogeny
  • pathway analysis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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