The nicotinic antagonist mecamylamine precipitates nicotine abstinence syndrome in the rat

David H. Malin, J. Ronald Lake, Victoria A. Carter, J. Scott Cunningham, Kathleen M. Hebert, Delia L. Conrad, Owen B. Wilson

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Recently, a rodent model of nicotine abstinence syndrome has been developed based on observing the frequency of spontaneous behavioral signs following termination of continuous subcutaneous infusion of nicotine tartrate. In the present study, the nicotinic antagonist mecamylamine precipitated an abstinence syndrome in nicotine-dependent rats. Twelve rats were each infused for 7 days with 9 mg/kg per day nicotine tartrate in saline via Alzet osmotic minipumps; another 12 rats were shamoperated and remained nicotine-naive. Six rats from each group received 1 mg/kg mecamylamine in saline SC immediately before a 30-min observation, while the remaining six rats from each group received saline alone. Nicotine-infused rats receiving mecamylamine exhibited significantly more (P<0.01), overall abstinence signs than all other groups. In terms of categories of signs, they displayed significantly more gasps/writhes, teeth chatter/chews, shakes/tremors and ptosis. In a second experiment utilizing only nicotine-naive rats, a far higher dose of mecamylamine (5 mg/kg sc) induced a quasi-nicotine abstinence syndrome. The results provide further validation for this rodent model of nicotine abstinence syndrome.

Original languageEnglish (US)
Pages (from-to)180-184
Number of pages5
JournalPsychopharmacology
Volume115
Issue number1-2
DOIs
StatePublished - Jun 1 1994

Keywords

  • Mecamylamine HCl
  • Nicotine
  • Nicotine abstinence
  • Nicotine dependence
  • Nicotinic receptor antagonists

ASJC Scopus subject areas

  • Pharmacology

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