The murine testicular transcriptome: Characterizing gene expression in the testis during the progression of spermatogenesis

James E. Shima, Derek J. McLean, John R. McCarrey, Michael D. Griswold

Research output: Contribution to journalArticlepeer-review

428 Scopus citations

Abstract

One of the most promising applications of microarrays is the study of changes in gene expression associated with the growth and development of mammalian tissues. The testis provides an excellent model to determine the ability of microarrays to effectively characterize the changes in gene expression as an organ develops from birth to adulthood. To this end, a developmental testis gene expression time course profiling the expression patterns of ∼36 000 transcripts on the Affymetrix MGU74v2 GeneChip platform at 11 distinct time points was created to gain a greater understanding of the molecular changes necessary for and elicited by the development of the testis. Additionally, gene expression profiles of isolated testicular cell types were created that can aid in the further characterization of the specific functional actions of each cell type in the testis. Statistical analysis of the data revealed 11 252 transcripts (9846 unique) expressed differentially in a significant manner. Subsequent cluster analysis produced five distinct expressional patterns within the time course. These patterns of expression are present at distinct chronological periods during testis development and often share similarities with cell-specific expression profiles. Analysis of cell-specific expression patterns produced unique and characteristic groups of transcripts that provide greater insight into the activities, biological and chronological, of testicular cell types during the progression of spermatogenesis. Further analysis of this time course can provide a distinct and more definitive view into the genes implicated, known and unknown, in the maturation, maintenance, and function of the testis and the integrated process of spermatogenesis.

Original languageEnglish (US)
Pages (from-to)319-330
Number of pages12
JournalBiology of reproduction
Volume71
Issue number1
DOIs
StatePublished - Jul 2004
Externally publishedYes

Keywords

  • Developmental biology
  • Gene regulation
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Cell Biology
  • Reproductive Medicine

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