The mouse βh1 gene codes for the z chain of embryonic hemoglobin

M. G. Farace; Betty A. Brown; Giuseppe Raschella; Jeff Alexanders; Roberto Gambari; Antonio Fantoni; Stephen C. Hardies; Clyde A. Hutchison; Marshall H. Edgell

The mouse βh1 gene codes for the z chain of embryonic hemoglobin

M. G. Farace, Betty A. Brown, Giuseppe Raschella, Jeff Alexanders, Roberto Gambari, Antonio Fantoni, Stephen C. Hardies, Clyde A. Hutchison, Marshall H. Edgell

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

βh0 and βh1 are two β-like globin genes in the mouse β-globin gene cluster whose functions have not previously been established. Transcripts of both βh0 and βh1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that βh1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a βh1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of βh1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected βh1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The βh1 translation product has the electrophoretic mobility expected for the z chain. We conclude that βh1 is a fully functional gene which codes for the embryonic z chain.

Original language	English (US)
Pages (from-to)	7123-7128
Number of pages	6
Journal	Journal of Biological Chemistry
Volume	259
Issue number	11
State	Published - 1984

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Access to Document

Fingerprint Dive into the research topics of 'The mouse βh1 gene codes for the z chain of embryonic hemoglobin'. Together they form a unique fingerprint.

Cite this

The mouse βh1 gene codes for the z chain of embryonic hemoglobin. / Farace, M. G.; Brown, Betty A.; Raschella, Giuseppe; Alexanders, Jeff; Gambari, Roberto; Fantoni, Antonio; Hardies, Stephen C.; Hutchison, Clyde A.; Edgell, Marshall H.

In: Journal of Biological Chemistry, Vol. 259, No. 11, 1984, p. 7123-7128.

Research output: Contribution to journal › Article › peer-review

@article{9361e2e321044b25b8da14de6dfa3f73,

title = "The mouse βh1 gene codes for the z chain of embryonic hemoglobin",

abstract = "βh0 and βh1 are two β-like globin genes in the mouse β-globin gene cluster whose functions have not previously been established. Transcripts of both βh0 and βh1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that βh1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a βh1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of βh1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected βh1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The βh1 translation product has the electrophoretic mobility expected for the z chain. We conclude that βh1 is a fully functional gene which codes for the embryonic z chain.",

author = "Farace, {M. G.} and Brown, {Betty A.} and Giuseppe Raschella and Jeff Alexanders and Roberto Gambari and Antonio Fantoni and Hardies, {Stephen C.} and Hutchison, {Clyde A.} and Edgell, {Marshall H.}",

year = "1984",

language = "English (US)",

volume = "259",

pages = "7123--7128",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "11",

}

TY - JOUR

T1 - The mouse βh1 gene codes for the z chain of embryonic hemoglobin

AU - Farace, M. G.

AU - Brown, Betty A.

AU - Raschella, Giuseppe

AU - Alexanders, Jeff

AU - Gambari, Roberto

AU - Fantoni, Antonio

AU - Hardies, Stephen C.

AU - Hutchison, Clyde A.

AU - Edgell, Marshall H.

PY - 1984

Y1 - 1984

N2 - βh0 and βh1 are two β-like globin genes in the mouse β-globin gene cluster whose functions have not previously been established. Transcripts of both βh0 and βh1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that βh1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a βh1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of βh1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected βh1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The βh1 translation product has the electrophoretic mobility expected for the z chain. We conclude that βh1 is a fully functional gene which codes for the embryonic z chain.

AB - βh0 and βh1 are two β-like globin genes in the mouse β-globin gene cluster whose functions have not previously been established. Transcripts of both βh0 and βh1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that βh1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a βh1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of βh1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected βh1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The βh1 translation product has the electrophoretic mobility expected for the z chain. We conclude that βh1 is a fully functional gene which codes for the embryonic z chain.

UR - http://www.scopus.com/inward/record.url?scp=0021233205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021233205&partnerID=8YFLogxK

M3 - Article

C2 - 6327707

AN - SCOPUS:0021233205

VL - 259

SP - 7123

EP - 7128

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 11

ER -