The mouse βh1 gene codes for the z chain of embryonic hemoglobin

M. G. Farace, Betty A. Brown, Giuseppe Raschella, Jeff Alexanders, Roberto Gambari, Antonio Fantoni, Stephen C. Hardies, Clyde A. Hutchison, Marshall H. Edgell

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

βh0 and βh1 are two β-like globin genes in the mouse β-globin gene cluster whose functions have not previously been established. Transcripts of both βh0 and βh1 are found in yolk sac-derived erythroid cells from mouse embryos and in the murine erythroleukemic cell line GM979. S1 nuclease analysis shows that βh1 is the more abundant of the two transcripts in both embryonic erythroid cells and the cell line GM979. Hybridization of a βh1-specific probe to RNA from erythroid cells of 10-, 11-, 12-, 13-, and 14-day-old mouse embryos indicates that levels of βh1 mRNA are high at 10 and 11 days and then decline during further fetal development. The in vitro translation product of hybrid-selected βh1 mRNA was analyzed by acid/urea polyacrylamide gel electrophoresis. The βh1 translation product has the electrophoretic mobility expected for the z chain. We conclude that βh1 is a fully functional gene which codes for the embryonic z chain.

Original languageEnglish (US)
Pages (from-to)7123-7128
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number11
StatePublished - 1984

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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