The molecular effects of alcohol: Clues to the enigmatic action of alcohol

Meena Kumari, Antje Anji, Henri Woods, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Chronic ethanol treatment (50 mM, five days) induces stabilization of NR1 receptor subunit mRNA in cultured fetal cortical neurons. In this paper, we investigate the mechanism(s) by which ethanol mediates its effects on NR1 mRNA. Specifically, we have determined if cellular localization of NR1 mRNA in cortical neurons and/or de novo protein synthesis is essential for ethanol-mediated stabilization of NR1 mRNA. Subcellular fractionation studies show that all detectable NR1 mRNA is associated with rough endoplasmic reticulum, indicating that subcellular distribution of NR1 mRNA in fetal cortical neurons does not play a role in ethanol-mediated NR1 mRNA stabilization. However, inhibition of protein synthesis by cycloheximide abolished the mRNA stabilizing effect of ethanol on NR1 mRNA, thus suggesting de novo protein synthesis is crucial for the action of ethanol on NR1 mRNA stabilization.

Original languageEnglish (US)
Pages (from-to)82-94
Number of pages13
JournalAnnals of the New York Academy of Sciences
Volume993
DOIs
StatePublished - 2003

Keywords

  • Chronic ethanol treatment
  • De novo protein synthesis
  • Fetal cortical neurons
  • mRNA stability
  • mRNA subcellular distribution
  • NMDA receptors
  • NR1 receptor subunit
  • Rough endoplasmic reticulum

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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