Abstract
The effect of added metals on purified rat tyrosine hydroxylase which is predominantly iron-free has been determined. The presence of 10 μM ferrous ammonium sulfate results in a ten-fold increase in the activity of enzyme containing 0.1 iron atom per subunit. The enzyme activity is half-maximal at a free ferrous iron concentration of 0.15 μM. Copper, zinc, silver, and nickel are unable to replace ferrous iron. Ferric iron is inactive unless ascorbate is included to reduce it.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 211-215 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 161 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 30 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The metal requirement of rat tyrosine hydroxylase. / Fitzpatrick, Paul F.
In: Biochemical and Biophysical Research Communications, Vol. 161, No. 1, 30.05.1989, p. 211-215.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The metal requirement of rat tyrosine hydroxylase
AU - Fitzpatrick, Paul F
PY - 1989/5/30
Y1 - 1989/5/30
N2 - The effect of added metals on purified rat tyrosine hydroxylase which is predominantly iron-free has been determined. The presence of 10 μM ferrous ammonium sulfate results in a ten-fold increase in the activity of enzyme containing 0.1 iron atom per subunit. The enzyme activity is half-maximal at a free ferrous iron concentration of 0.15 μM. Copper, zinc, silver, and nickel are unable to replace ferrous iron. Ferric iron is inactive unless ascorbate is included to reduce it.
AB - The effect of added metals on purified rat tyrosine hydroxylase which is predominantly iron-free has been determined. The presence of 10 μM ferrous ammonium sulfate results in a ten-fold increase in the activity of enzyme containing 0.1 iron atom per subunit. The enzyme activity is half-maximal at a free ferrous iron concentration of 0.15 μM. Copper, zinc, silver, and nickel are unable to replace ferrous iron. Ferric iron is inactive unless ascorbate is included to reduce it.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024324003&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)91582-9
DO - 10.1016/0006-291X(89)91582-9
M3 - Article
VL - 161
SP - 211
EP - 215
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -