The membrane potential of Ehrlich ascites tumor cells: An evaluation of the null point method

Thomas C. Smith, Susan C. Robinson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The effects of valinomycin (25 pM) on the membrane potential and on initial, passive Na+ and K+ movements have been determined in Ehrlich ascites tumor cells. The membrane potential of steady‐state cells in a physiologic environment was – 23.2 mV. Addition of valinomycin induced a small, significant hyperpolarization (Vm = –29.6 mV) when averaged over the population tested. However, analyses of the response of individual cells to valinomycin showed two different potential effects: (1) the majority of cells hyperpolarized after treatment; but (2) a significant fraction depolarized when exposed to valinomycin. The Vm of steady‐state cells incubated in saline with K+ at concentrations of 21 mM or 75 mM was – 21.4 mV and –22.0 mV, respectively. Addition of valinomycin to these cells was without effect on Vm, thus establishing the “null point” responses. Only for cells incubated in saline with a K+ of 75 mM was there agreement between Vm and K+ equilibrium potential (Vk). Determinations of cellular Na+ and K+ showed that valinomycin induced net losses of K+ and gains of Na+ by cells incubated in either physiologic saline or saline with a K+ concentration of 21 mM. However, the celular K+ of cells incubated in saline with a K+ concentration of 75 mM was unaltered by valinomycin. There was a two‐ to threefold increase in K+ permeability of the cell membrane in the presence of valinomycin. These results are consistent with the existence of two null points in the membrane‐potential response to valinomycin: One is established when the membrane potential corresponds to Vk; the second occurs when the effects of valinomycin on K+ loss from the cell are exactly offset by its inhibition of active Na+ + K+ transport.

Original languageEnglish (US)
Pages (from-to)399-406
Number of pages8
JournalJournal of Cellular Physiology
Volume106
Issue number3
DOIs
StatePublished - Mar 1981
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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