The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis

Kokichi Hanaoka, Dongxu Sun, Richard Lawrence, Yoshinori Kamitani, Gabriel Fernandes

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We reported that reduced water produced by electrolysis enhanced the antioxidant effects of proton donors such as ascorbic acid (AsA) in a previous paper. We also demonstrated that reduced water produced by electrolysis of 2 mM NaCl solutions did not show antioxidant effects by itself. We reasoned that the enhancement of antioxidant effects may be due to the increase of the ionic product of water as solvent. The ionic product of water (pKw) was estimated by measurements of pH and by a neutralization titration method. As an indicator of oxidative damage, Reactive Oxygen Species- (ROS) mediated DNA strand breaks were measured by the conversion of supercoiled φX-174 RF I double-strand DNA to open and linear forms. Reduced water had a tendency to suppress single-strand breakage of DNA induced by reactive oxygen species produced by H2O2/Cu (II) and HQ/Cu (II) systems. The enhancement of superoxide anion radical dismutation activity can be explained by changes in the ionic product of water in the reduced water.

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalBiophysical Chemistry
Volume107
Issue number1
DOIs
StatePublished - Jan 1 2004

Keywords

  • Antioxidant
  • DNA damage
  • Ionic product of water
  • Reduced water

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis'. Together they form a unique fingerprint.

  • Cite this