@article{baad81b08354439d99046bcab621258f,
title = "The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids",
abstract = "Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduction and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermatogenic cell types, the full continuum of gene expression patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we catalog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, elucidate the full range of gene expression changes during male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contraceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve spermatogenesis in vitro. Hermann et al. present single-cell transcriptomes from >62,000 individual spermatogenic cells from immature and adult male mice and adult men. Their analysis facilitates resolution of SSCs and progenitor spermatogonia, elucidates the full range of gene expression changes during male meiosis and spermiogenesis, and derives unique gene expression signatures for eleven mouse and human spermatogenic cell types.",
keywords = "RNA, continuum, heterogeneity, human, male germ cells, meiosis, mouse, spermiogenesis",
author = "Hermann, {Brian P.} and Keren Cheng and Anukriti Singh and {Roa-De La Cruz}, Lorena and Mutoji, {Kazadi N.} and Chen, {I. Chung} and Heidi Gildersleeve and Lehle, {Jake D.} and Max Mayo and Birgit Westernstr{\"o}er and Law, {Nathan C.} and Oatley, {Melissa J.} and Velte, {Ellen K.} and Niedenberger, {Bryan A.} and Danielle Fritze and Sherman Silber and Geyer, {Christopher B.} and Oatley, {Jon M.} and McCarrey, {John R.}",
note = "Funding Information: The authors are grateful for computational support from Armando Rodiguez and Jeremy Mann in the UTSA Research Computing Support Group, as well as the Shamu HPC cluster operated by the UTSA Office of Information Technology. Sequencing data were generated by Chaoying Liang and Kasthuribai Viswanathan in the UT Southwestern Genomics and Microarray Core, as well as Dawn Garcia and Zhou Lai from the Genome Sequencing Facility (GSF) at Greehey Children{\textquoteright}s Cancer Research Institute in the UT Health San Antonio (UTHSA). The graphical abstract was produced by Victoria Foor. This work was supported by (in no particular order) NIH grants P30-CA054174 (UTHSA), P30-GM092334 (J.R.M.), R01-HD061665 (J.M.O.), R00-HD062687 (B.P.H.), R01-HD078679 (J.R.M.), R01-HD90007 (B.P.H.), R01-HD90083 (C.B.G.), G12-MD007591 (UTHSA), S10-OD021805 (UTHSA), and UL1-RR025767 (UTHSA); NSF grant DBI-1337513 (B.P.H.); CPRIT Core Facility Award RP160732 (UTHSA); a fellowship from the Deutsches Primatenzentrum (B.W.); a UTHSA IIMS/CTSA Translational Technology Supplement (B.P.H.); the Max and Minnie Tomerlin Voelcker Fund (B.P.H.); the Helen Freeborn Kerr Charitable Foundation (B.P.H.); and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation (J.R.M.). Funding Information: The authors are grateful for computational support from Armando Rodiguez and Jeremy Mann in the UTSA Research Computing Support Group, as well as the Shamu HPC cluster operated by the UTSA Office of Information Technology. Sequencing data were generated by Chaoying Liang and Kasthuribai Viswanathan in the UT Southwestern Genomics and Microarray Core, as well as Dawn Garcia and Zhou Lai from the Genome Sequencing Facility (GSF) at Greehey Children's Cancer Research Institute in the UT Health San Antonio (UTHSA). The graphical abstract was produced by Victoria Foor. This work was supported by (in no particular order) NIH grants P30-CA054174 (UTHSA), P30-GM092334 (J.R.M.), R01-HD061665 (J.M.O.), R00-HD062687 (B.P.H.), R01-HD078679 (J.R.M.), R01-HD90007 (B.P.H.), R01-HD90083 (C.B.G.), G12-MD007591 (UTHSA), S10-OD021805 (UTHSA), and UL1-RR025767 (UTHSA); NSF grant DBI-1337513 (B.P.H.); CPRIT Core Facility Award RP160732 (UTHSA); a fellowship from the Deutsches Primatenzentrum (B.W.); a UTHSA IIMS/CTSA Translational Technology Supplement (B.P.H.); the Max and Minnie Tomerlin Voelcker Fund (B.P.H.); the Helen Freeborn Kerr Charitable Foundation (B.P.H.); and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation (J.R.M.). Publisher Copyright: {\textcopyright} 2018 The Author(s)",
year = "2018",
month = nov,
day = "6",
doi = "10.1016/j.celrep.2018.10.026",
language = "English (US)",
volume = "25",
pages = "1650--1667.e8",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "6",
}