Human apolipoprotein (apo) C-II, a 79 amino acid protein, functions as a cofactor for lipoprotein lipase, the enzyme which catalyzes the hydrolysis of plasma triglycerides. The chromosomal location of apoC-II has been determined by filter hybridization analysis of human-mouse hybrid cells. Southern blots of DNA from 21 human-mouse hybrid cells were hybridized with a 190 base pair nick translated probe prepared from a Hinf I digest of an apoC-II cDNA clone. Without exception, ApoC-II segregated with chromosome 19 thus establishing synteny with the apoE and LDL receptor genes known to be localized to this chromosome. The localization of the apoC-II gene to chromosome 19 will permit more detailed analysis of the genomic organization and linkages of the apolipoprotein genes.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 31 1984|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology