THE LEVELS OF LABILE INTERMEDIARY METABOLITES IN MOUSE BRAIN FOLLOWING RAPID TISSUE FIXATION WITH MICROWAVE IRRADIATION

M. A. Medina; D. J. Jones; W. B. Stavinoha; D. H. Ross

doi:10.1111/j.1471-4159.1975.tb11868.x

THE LEVELS OF LABILE INTERMEDIARY METABOLITES IN MOUSE BRAIN FOLLOWING RAPID TISSUE FIXATION WITH MICROWAVE IRRADIATION

M. A. Medina, D. J. Jones, W. B. Stavinoha, D. H. Ross

Anesthesiology

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

The levels of several labile glycolytic and organic phosphate metabolites in mouse brain were determined following rapid inactivation with 2450 MHz microwave irradiation. The levels of ATP in mouse brain following a 0·25 s exposure in a 6 kW microwave oven was found to be 2·416 ± 0·061. Whole brain levels of 8 labile intermediary metabolites in 0·4 s irradiated samples were comparable to those reported using the previously‐described methods of freeze‐blowing or whole‐animal immersion. Analysis of these same metabolites in 4 gross areas of brain did not reveal any anoxic changes betwen superficial and deeper brain areas. The advantages of the mcrowave irradiation inactivation technique for regional brain studies of labile intermediary metabolites is discussed.

Original language	English (US)
Pages (from-to)	223-227
Number of pages	5
Journal	Journal of Neurochemistry
Volume	24
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-4159.1975.tb11868.x
State	Published - Feb 1975

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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abstract = "The levels of several labile glycolytic and organic phosphate metabolites in mouse brain were determined following rapid inactivation with 2450 MHz microwave irradiation. The levels of ATP in mouse brain following a 0·25 s exposure in a 6 kW microwave oven was found to be 2·416 ± 0·061. Whole brain levels of 8 labile intermediary metabolites in 0·4 s irradiated samples were comparable to those reported using the previously‐described methods of freeze‐blowing or whole‐animal immersion. Analysis of these same metabolites in 4 gross areas of brain did not reveal any anoxic changes betwen superficial and deeper brain areas. The advantages of the mcrowave irradiation inactivation technique for regional brain studies of labile intermediary metabolites is discussed.",

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