The large viral RNA segment of California serogroup Bunyaviruses encodes the large viral protein

M. J. Endres, D. R. Jacoby, R. S. Janssen, F. Gonzalez-Scarano, N. Nathanson

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Reassortant bunyaviruses derived from two members of the California serogroup (La Crosse/original and Tahyna/181-57) viruses were used to demonstrate that the large M(r) viral protein (L) is encoded by the L RNA segment. Radiolabelled viral proteins were analysed by discontinuous SDS-PAGE. The L protein of La Crosse virus was observed to migrate ahead of its Tahyna virus counterpart when electrophoresed through a 5% acrylamide resolving gel. Among the reassortant viruses, the L protein phenotype segregated with the viral L RNA segment. After confirming the genotype of the viruses used in this study, it was concluded that the L RNA species of California serogroup viruses codes for the L protein, the presumed viral polymerase.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalJournal of General Virology
Volume70
Issue number1
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Virology

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    Endres, M. J., Jacoby, D. R., Janssen, R. S., Gonzalez-Scarano, F., & Nathanson, N. (1989). The large viral RNA segment of California serogroup Bunyaviruses encodes the large viral protein. Journal of General Virology, 70(1), 223-228. https://doi.org/10.1099/0022-1317-70-1-223