The interactions of LIM protein KyoT with polycomb proteins

LIM domain protein KyoT interacts with transcription factor RBP-J and modulates Notch signaling pathway. To study the function of KyoT, yeast two-hybrid was performed and proteins interacting with KyoT2 including Ring1 and hPc2 (human polycomb 2) that belong to PcG (polycomb group) were analyzed. The interactions between Ring1, hPc2, and KyoT2 were confirmed by changing vectors in two-hybrid analysis. Their interactions in vitro were confirmed by GST-pull down assay. Two-hybrid assay showed that the LIM domain of KyoT was responsible for interacting with the full-length Ring1 and hPc2. Moreover, the LIM domain also interacted with the C-terminus of hPc2. Interactions of KyoT2 with Ring1 and hPc2 suggest that PcG family may be involved in Notch signaling pathway.