When human α2 macroglobulin (α2M) or its asialo-[3H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α2M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jan 29 1980|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology