The interaction of α2 macroglobulin with trypsin releases a soluble glycopeptide

Y. T. Pan; A. K. Mukherjee; Paul M. Horowitz; Alan D. Elbein

doi:10.1016/0006-291X(80)90390-3

The interaction of α₂ macroglobulin with trypsin releases a soluble glycopeptide

Y. T. Pan, A. K. Mukherjee, Paul M. Horowitz, Alan D. Elbein

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

When human α₂ macroglobulin (α₂M) or its asialo-[³H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α₂M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.

Original language	English (US)
Pages (from-to)	703-709
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	92
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)90390-3
State	Published - Jan 29 1980
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(80)90390-3

Cite this

@article{8257452880d5452a948b30d1634448c0,

title = "The interaction of α2 macroglobulin with trypsin releases a soluble glycopeptide",

abstract = "When human α2 macroglobulin (α2M) or its asialo-[3H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α2M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.",

author = "Pan, {Y. T.} and Mukherjee, {A. K.} and Horowitz, {Paul M.} and Elbein, {Alan D.}",

note = "Funding Information: This work was supported by grant AM 21800 from the National Institute of Arthritis and Metabolic Diseases and Grant 77-08801 from the National Science Foundation to Alan D. Elbein and Grant GM 25177 from the National Institute of Health and Welch Grant AQ 723 to Paul M. Horowitz.",

year = "1980",

month = jan,

day = "29",

doi = "10.1016/0006-291X(80)90390-3",

language = "English (US)",

volume = "92",

pages = "703--709",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - The interaction of α2 macroglobulin with trypsin releases a soluble glycopeptide

AU - Pan, Y. T.

AU - Mukherjee, A. K.

AU - Horowitz, Paul M.

AU - Elbein, Alan D.

N1 - Funding Information: This work was supported by grant AM 21800 from the National Institute of Arthritis and Metabolic Diseases and Grant 77-08801 from the National Science Foundation to Alan D. Elbein and Grant GM 25177 from the National Institute of Health and Welch Grant AQ 723 to Paul M. Horowitz.

PY - 1980/1/29

Y1 - 1980/1/29

N2 - When human α2 macroglobulin (α2M) or its asialo-[3H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α2M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.

AB - When human α2 macroglobulin (α2M) or its asialo-[3H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α2M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.

UR - http://www.scopus.com/inward/record.url?scp=0019334858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019334858&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(80)90390-3

DO - 10.1016/0006-291X(80)90390-3

M3 - Article

C2 - 6153527

AN - SCOPUS:0019334858

VL - 92

SP - 703

EP - 709

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

The interaction of α₂ macroglobulin with trypsin releases a soluble glycopeptide

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this