The interaction of α2 macroglobulin with trypsin releases a soluble glycopeptide

Y. T. Pan, A. K. Mukherjee, Paul M. Horowitz, Alan D. Elbein

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

When human α2 macroglobulin (α2M) or its asialo-[3H]galactose derivative reacts with trypsin, a glycopeptide of molecular weight 3500-4000 is released from the α2M. The glycopeptide was purified on Biogel P-4 columns and its amino acid and carbohydrate composition were determined. The oligosaccharide contains sialic acid, galactose, mannose and GlcNAc in a ratio of 1.0:0.73:3.85:2.85 and is apparently attached to protein in a GlcNAc→asparagine linkage.

Original languageEnglish (US)
Pages (from-to)703-709
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume92
Issue number2
DOIs
StatePublished - Jan 29 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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