The inability of in vitro transforming SV40 subgenomes to cause tumors in vivo

Mary Pat Moyer; Helen Arizpe; Aurora Sosa; Katia Pierson; Rex C. Moyer

doi:10.1159/000149506

The inability of in vitro transforming SV40 subgenomes to cause tumors in vivo

Mary Pat Moyer, Helen Arizpe, Aurora Sosa, Katia Pierson, Rex C. Moyer

Research output: Contribution to journal › Article › peer-review

Abstract

Linear or subgenomic SV40 DNAs were transfected into cells from a variety of species (including rodent, dog, muntjak, and monkey) and injected subcutaneously into neonate Syrian hamsters for tumorigenicity testing. The 'early-region' subgenomes were capable of transforming cells in vitro. Complete genomes or complementary subgenomes could transform non- permissive and semipermissive cells, were infectious for permissive cells, and induced tumors from which infectious virus could be rescued. Tumors were not formed in neonate hamsters upon injection with subgenomic SV40 DNAs, even those capable of transforming cells in vitro. These results suggested that SV40 tumor formation in vivo may require a complete genome.

Original language	English (US)
Pages (from-to)	87-95
Number of pages	9
Journal	Intervirology
Volume	21
Issue number	2
DOIs	https://doi.org/10.1159/000149506
State	Published - 1984

Keywords

Papovaviruses
Recombinant DNA techniques
Restriction enzymes
SV40
Transformation
Tumorigenesis
Virus production

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1159/000149506

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title = "The inability of in vitro transforming SV40 subgenomes to cause tumors in vivo",

abstract = "Linear or subgenomic SV40 DNAs were transfected into cells from a variety of species (including rodent, dog, muntjak, and monkey) and injected subcutaneously into neonate Syrian hamsters for tumorigenicity testing. The 'early-region' subgenomes were capable of transforming cells in vitro. Complete genomes or complementary subgenomes could transform non- permissive and semipermissive cells, were infectious for permissive cells, and induced tumors from which infectious virus could be rescued. Tumors were not formed in neonate hamsters upon injection with subgenomic SV40 DNAs, even those capable of transforming cells in vitro. These results suggested that SV40 tumor formation in vivo may require a complete genome.",

keywords = "Papovaviruses, Recombinant DNA techniques, Restriction enzymes, SV40, Transformation, Tumorigenesis, Virus production",

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AU - Moyer, Mary Pat

AU - Arizpe, Helen

AU - Sosa, Aurora

AU - Pierson, Katia

AU - Moyer, Rex C.

PY - 1984

Y1 - 1984

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AB - Linear or subgenomic SV40 DNAs were transfected into cells from a variety of species (including rodent, dog, muntjak, and monkey) and injected subcutaneously into neonate Syrian hamsters for tumorigenicity testing. The 'early-region' subgenomes were capable of transforming cells in vitro. Complete genomes or complementary subgenomes could transform non- permissive and semipermissive cells, were infectious for permissive cells, and induced tumors from which infectious virus could be rescued. Tumors were not formed in neonate hamsters upon injection with subgenomic SV40 DNAs, even those capable of transforming cells in vitro. These results suggested that SV40 tumor formation in vivo may require a complete genome.

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KW - Transformation

KW - Tumorigenesis

KW - Virus production

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The inability of in vitro transforming SV40 subgenomes to cause tumors in vivo

Abstract

Keywords

ASJC Scopus subject areas

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