The in vitro effect of Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide on murine peritoneal macrophages.

K. A. Barker, S. C. Holt

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide (LPS) was examined for its in vitro effect on murine peritoneal macrophage morphology, viability, and lysosomal enzyme activity. Fifty micrograms of Y4 LPS per 10(6) macrophages resulted in macrophage activation, eliciting the release of acid phosphates (AcP), as well as the accumulation of intracellular AcP, without a loss in viability. There was also an increase in the number of organelles and cell-surface ruffles. One hundred or 250 microgram of Y4 LPS caused flattening, rounding, and blebbing of the cell surface, as well as the release of large quantities of AcP and lactate dehydrogenase (LDH), within 5 min of exposure to the LPS. Alteration of cell ultrastructure occurred within 30 min, and extensive autophagocytosis by 24 h, indicative of cell injury. Short-term (less than 24 h) experiments appear necessary to distinguish between the effects of cell activation and cell death caused by A. actinomycetemcomitans Y4 LPS.

Original languageEnglish (US)
Pages (from-to)1552-1563
Number of pages12
JournalCanadian journal of microbiology
Volume29
Issue number11
DOIs
StatePublished - Nov 1983
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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