Abstract
The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The 'A' gene1, also known as Q2 P'3, ORF-14 or sor 5, partially overlaps the pol gene; its protein product has a relative molecular mass of 23,000 (Mr 23K) and is present in productively infected cells7-10. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4+ lymphocyte lines8, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-211 and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the 'A' gene which produces virion particles normally; however, the particles are & sm;1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4+ lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the 'A' gene product and probably does not require the production of infective virus particles.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 728-730 |
| Number of pages | 3 |
| Journal | Nature |
| Volume | 328 |
| Issue number | 6132 |
| State | Published - 1988 |
| Externally published | Yes |
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The HIV A (sor) gene product is essential for virus infectivity. / Strebel, Klaus; Daugherty, Daryl; Clouse, Kathleen; Cohen, David; Folks, Tom; Martin, Malcolm A.
In: Nature, Vol. 328, No. 6132, 1988, p. 728-730.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The HIV A (sor) gene product is essential for virus infectivity
AU - Strebel, Klaus
AU - Daugherty, Daryl
AU - Clouse, Kathleen
AU - Cohen, David
AU - Folks, Tom
AU - Martin, Malcolm A.
PY - 1988
Y1 - 1988
N2 - The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The 'A' gene1, also known as Q2 P'3, ORF-14 or sor 5, partially overlaps the pol gene; its protein product has a relative molecular mass of 23,000 (Mr 23K) and is present in productively infected cells7-10. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4+ lymphocyte lines8, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-211 and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the 'A' gene which produces virion particles normally; however, the particles are & sm;1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4+ lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the 'A' gene product and probably does not require the production of infective virus particles.
AB - The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The 'A' gene1, also known as Q2 P'3, ORF-14 or sor 5, partially overlaps the pol gene; its protein product has a relative molecular mass of 23,000 (Mr 23K) and is present in productively infected cells7-10. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4+ lymphocyte lines8, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-211 and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the 'A' gene which produces virion particles normally; however, the particles are & sm;1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4+ lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the 'A' gene product and probably does not require the production of infective virus particles.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023267788&partnerID=8YFLogxK
M3 - Article
C2 - 2441266
AN - SCOPUS:0023267788
VL - 328
SP - 728
EP - 730
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6132
ER -