The heat shock transcription factor in liver exists in a form that has DNA binding activity but no transcriptional activity

Ryoya Takahashi, Ahmad R. Heydari, Astrid Gutsmann, Marian Sabia, Arlan Richardson

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The binding activity of the heat shock transcription factor (HSF) to the heat shock element (HSE) is observed in non-stressed liver and freshly isolated hepatocytes when the expression of hsp70 is undetectable. HSF binding activity in non-stressed liver/hepatocytes is specific for HSE and similar to the HSF binding activity observed in heat shocked hepatocytes that is associated with hsp70 transcription. However, the HSF binding activity in non-stressed and heat shock cells can be distinguished on the basis of the thermal stability in vitro. The HSE binding activity of cell extracts isolated from non-stressed liver/hepatocytes was lost rapidly when the extracts were incubated at 37°C. In contrast, the HSF binding activity of cell extracts isolated from heat shocked hepatocytes was relatively stable at 37°C. Based on our observations, we propose that the activation of HSF is a multistep process that involves a change in conformation after oligomerization and the acquisition of DNA binding to a form that is more thermostable and is associated with increased hsp70 transcription.

Original languageEnglish (US)
Pages (from-to)552-558
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number2
DOIs
StatePublished - Jun 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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