The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans

Brendan J. Loftus, Eula Fung, Paola Roncaglia, Don Rowley, Paolo Amedeo, Dan Bruno, Jessica Vamathevan, Molly Miranda, Iain J. Anderson, James A. Fraser, Jonathan E. Allen, Ian E. Bosdet, Michael R. Brent, Readman Chiu, Tamara L. Doering, Maureen J. Donlin, Cletus A. D'Souza, Deborah S. Fox, Viktoriya Grinberg, Jianmin FuMarilyn Fukushima, Brian J. Haas, James C. Huang, Guilhem Janbon, Steven J M Jones, Hean L. Koo, Martin I. Krzywinski, June K. Kwon-Chung, Klaus B. Lengeler, Rama Maiti, Marco A. Marra, Robert E. Marra, Carrie A. Mathewson, Thomas G. Mitchell, Mihaela Pertea, Florenta R. Riggs, Steven L. Salzberg, Jacqueline E. Schein, Alla Shvartsbeyn, Heesun Shin, Martin Shumway, Charles A. Specht, Bernard B. Suh, Aaron Tenney, Terry R. Utterback, Brian L Wickes, Jennifer R. Wortman, Natasja H. Wye, James W. Kronstad, Jennifer K. Lodge, Joseph Heitman, Ronald W. Davis, Claire M. Fraser, Richard W. Hyman

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460 Citations (Scopus)

Abstract

Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.

Original languageEnglish (US)
Pages (from-to)1321-1324
Number of pages4
JournalScience
Volume307
Issue number5713
DOIs
StatePublished - Feb 25 2005

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Cryptococcus neoformans
Yeasts
Genome
Genes
Karyotype
Transcriptome
Introns
Virulence

ASJC Scopus subject areas

  • General

Cite this

Loftus, B. J., Fung, E., Roncaglia, P., Rowley, D., Amedeo, P., Bruno, D., ... Hyman, R. W. (2005). The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science, 307(5713), 1321-1324. https://doi.org/10.1126/science.1103773

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. / Loftus, Brendan J.; Fung, Eula; Roncaglia, Paola; Rowley, Don; Amedeo, Paolo; Bruno, Dan; Vamathevan, Jessica; Miranda, Molly; Anderson, Iain J.; Fraser, James A.; Allen, Jonathan E.; Bosdet, Ian E.; Brent, Michael R.; Chiu, Readman; Doering, Tamara L.; Donlin, Maureen J.; D'Souza, Cletus A.; Fox, Deborah S.; Grinberg, Viktoriya; Fu, Jianmin; Fukushima, Marilyn; Haas, Brian J.; Huang, James C.; Janbon, Guilhem; Jones, Steven J M; Koo, Hean L.; Krzywinski, Martin I.; Kwon-Chung, June K.; Lengeler, Klaus B.; Maiti, Rama; Marra, Marco A.; Marra, Robert E.; Mathewson, Carrie A.; Mitchell, Thomas G.; Pertea, Mihaela; Riggs, Florenta R.; Salzberg, Steven L.; Schein, Jacqueline E.; Shvartsbeyn, Alla; Shin, Heesun; Shumway, Martin; Specht, Charles A.; Suh, Bernard B.; Tenney, Aaron; Utterback, Terry R.; Wickes, Brian L; Wortman, Jennifer R.; Wye, Natasja H.; Kronstad, James W.; Lodge, Jennifer K.; Heitman, Joseph; Davis, Ronald W.; Fraser, Claire M.; Hyman, Richard W.

In: Science, Vol. 307, No. 5713, 25.02.2005, p. 1321-1324.

Research output: Contribution to journalArticle

Loftus, BJ, Fung, E, Roncaglia, P, Rowley, D, Amedeo, P, Bruno, D, Vamathevan, J, Miranda, M, Anderson, IJ, Fraser, JA, Allen, JE, Bosdet, IE, Brent, MR, Chiu, R, Doering, TL, Donlin, MJ, D'Souza, CA, Fox, DS, Grinberg, V, Fu, J, Fukushima, M, Haas, BJ, Huang, JC, Janbon, G, Jones, SJM, Koo, HL, Krzywinski, MI, Kwon-Chung, JK, Lengeler, KB, Maiti, R, Marra, MA, Marra, RE, Mathewson, CA, Mitchell, TG, Pertea, M, Riggs, FR, Salzberg, SL, Schein, JE, Shvartsbeyn, A, Shin, H, Shumway, M, Specht, CA, Suh, BB, Tenney, A, Utterback, TR, Wickes, BL, Wortman, JR, Wye, NH, Kronstad, JW, Lodge, JK, Heitman, J, Davis, RW, Fraser, CM & Hyman, RW 2005, 'The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans', Science, vol. 307, no. 5713, pp. 1321-1324. https://doi.org/10.1126/science.1103773
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D et al. The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science. 2005 Feb 25;307(5713):1321-1324. https://doi.org/10.1126/science.1103773
Loftus, Brendan J. ; Fung, Eula ; Roncaglia, Paola ; Rowley, Don ; Amedeo, Paolo ; Bruno, Dan ; Vamathevan, Jessica ; Miranda, Molly ; Anderson, Iain J. ; Fraser, James A. ; Allen, Jonathan E. ; Bosdet, Ian E. ; Brent, Michael R. ; Chiu, Readman ; Doering, Tamara L. ; Donlin, Maureen J. ; D'Souza, Cletus A. ; Fox, Deborah S. ; Grinberg, Viktoriya ; Fu, Jianmin ; Fukushima, Marilyn ; Haas, Brian J. ; Huang, James C. ; Janbon, Guilhem ; Jones, Steven J M ; Koo, Hean L. ; Krzywinski, Martin I. ; Kwon-Chung, June K. ; Lengeler, Klaus B. ; Maiti, Rama ; Marra, Marco A. ; Marra, Robert E. ; Mathewson, Carrie A. ; Mitchell, Thomas G. ; Pertea, Mihaela ; Riggs, Florenta R. ; Salzberg, Steven L. ; Schein, Jacqueline E. ; Shvartsbeyn, Alla ; Shin, Heesun ; Shumway, Martin ; Specht, Charles A. ; Suh, Bernard B. ; Tenney, Aaron ; Utterback, Terry R. ; Wickes, Brian L ; Wortman, Jennifer R. ; Wye, Natasja H. ; Kronstad, James W. ; Lodge, Jennifer K. ; Heitman, Joseph ; Davis, Ronald W. ; Fraser, Claire M. ; Hyman, Richard W. / The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. In: Science. 2005 ; Vol. 307, No. 5713. pp. 1321-1324.
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abstract = "Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its ∼20-megabase genome, which contains ∼6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.",
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AU - Roncaglia, Paola

AU - Rowley, Don

AU - Amedeo, Paolo

AU - Bruno, Dan

AU - Vamathevan, Jessica

AU - Miranda, Molly

AU - Anderson, Iain J.

AU - Fraser, James A.

AU - Allen, Jonathan E.

AU - Bosdet, Ian E.

AU - Brent, Michael R.

AU - Chiu, Readman

AU - Doering, Tamara L.

AU - Donlin, Maureen J.

AU - D'Souza, Cletus A.

AU - Fox, Deborah S.

AU - Grinberg, Viktoriya

AU - Fu, Jianmin

AU - Fukushima, Marilyn

AU - Haas, Brian J.

AU - Huang, James C.

AU - Janbon, Guilhem

AU - Jones, Steven J M

AU - Koo, Hean L.

AU - Krzywinski, Martin I.

AU - Kwon-Chung, June K.

AU - Lengeler, Klaus B.

AU - Maiti, Rama

AU - Marra, Marco A.

AU - Marra, Robert E.

AU - Mathewson, Carrie A.

AU - Mitchell, Thomas G.

AU - Pertea, Mihaela

AU - Riggs, Florenta R.

AU - Salzberg, Steven L.

AU - Schein, Jacqueline E.

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AU - Suh, Bernard B.

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AU - Utterback, Terry R.

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AU - Wortman, Jennifer R.

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AU - Lodge, Jennifer K.

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