The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells

Joseph Mazar, Wei Zhao, Ahmad M. Khalil, Bongyong Lee, John Shelley, Subramaniam S. Govindarajan, Fumiko Yamamoto, Maya Ratnam, Muhammad Nauman Aftab, Sheila Collins, Brian N. Finck, Xianlin Han, John S. Mattick, Marcel E. Dinger, Ranjan J. Perera

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Expression of the long noncoding RNA (lncRNA) SPRY4-IT1 is low in normal human melanocytes but high in melanoma cells. siRNA knockdown of SPRY4-IT1 blocks melanoma cell invasion and proliferation, and increases apoptosis. To investigate its function further, we affinity purified SPRY4-IT1 from melanoma cells and used mass spectrometry to identify the protein lipin 2, an enzyme that converts phosphatidate to diacylglycerol (DAG), as a major binding partner. SPRY4-IT1 knockdown increases the accumulation of lipin2 protein and upregulate the expression of diacylglycerol O-acyltransferase 2 (DGAT2) an enzyme involved in the conversion of DAG to triacylglycerol (TAG). When SPRY4-IT1 knockdown and control melanoma cells were subjected to shotgun lipidomics, an MS-based assay that permits the quantification of changes in the cellular lipid profile, we found that SPRY4-IT1 knockdown induced significant changes in a number of lipid species, including increased acyl carnitine, fatty acyl chains, and triacylglycerol (TAG). Together, these results suggest the possibility that SPRY4-IT1 knockdown may induce apoptosis via lipin 2-mediated alterations in lipid metabolism leading to cellular lipotoxicity.

Original languageEnglish (US)
Pages (from-to)8959-8969
Number of pages11
JournalOncotarget
Volume5
Issue number19
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Melanoma
Diglycerides
Triglycerides
Diacylglycerol O-Acyltransferase
Long Noncoding RNA
Apoptosis
Lipids
Carnitine
Melanocytes
Firearms
Enzymes
Lipid Metabolism
Small Interfering RNA
Mass Spectrometry
Proteins
Up-Regulation
Cell Proliferation
human long noncoding RNA SPRY4-IT1
lipine

Keywords

  • Long noncoding RNA
  • Melanoma

ASJC Scopus subject areas

  • Oncology

Cite this

Mazar, J., Zhao, W., Khalil, A. M., Lee, B., Shelley, J., Govindarajan, S. S., ... Perera, R. J. (2014). The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells. Oncotarget, 5(19), 8959-8969. https://doi.org/10.18632/oncotarget.1863

The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells. / Mazar, Joseph; Zhao, Wei; Khalil, Ahmad M.; Lee, Bongyong; Shelley, John; Govindarajan, Subramaniam S.; Yamamoto, Fumiko; Ratnam, Maya; Aftab, Muhammad Nauman; Collins, Sheila; Finck, Brian N.; Han, Xianlin; Mattick, John S.; Dinger, Marcel E.; Perera, Ranjan J.

In: Oncotarget, Vol. 5, No. 19, 01.01.2014, p. 8959-8969.

Research output: Contribution to journalArticle

Mazar, J, Zhao, W, Khalil, AM, Lee, B, Shelley, J, Govindarajan, SS, Yamamoto, F, Ratnam, M, Aftab, MN, Collins, S, Finck, BN, Han, X, Mattick, JS, Dinger, ME & Perera, RJ 2014, 'The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells', Oncotarget, vol. 5, no. 19, pp. 8959-8969. https://doi.org/10.18632/oncotarget.1863
Mazar J, Zhao W, Khalil AM, Lee B, Shelley J, Govindarajan SS et al. The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells. Oncotarget. 2014 Jan 1;5(19):8959-8969. https://doi.org/10.18632/oncotarget.1863
Mazar, Joseph ; Zhao, Wei ; Khalil, Ahmad M. ; Lee, Bongyong ; Shelley, John ; Govindarajan, Subramaniam S. ; Yamamoto, Fumiko ; Ratnam, Maya ; Aftab, Muhammad Nauman ; Collins, Sheila ; Finck, Brian N. ; Han, Xianlin ; Mattick, John S. ; Dinger, Marcel E. ; Perera, Ranjan J. / The functional characterization of long noncoding RNA SPRY4-IT1 in human melanoma cells. In: Oncotarget. 2014 ; Vol. 5, No. 19. pp. 8959-8969.
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