The F-box protein rcy1 is involved in the degradation of histone H3 variant Cse4 and genome maintenance

Haili Cheng, Xin Bao, Hai Rao

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Cse4, a histone H3-like centromeric protein, plays critical functions in chromosome segregation. Cse4 level is tightly regulated, but the underlying mechanism remains poorly understood. We employed a toxicity-based screen to look for the degradation components involved in Cse4 regulation. Here, we show that the F-box containing protein Rcy1 is required for efficient Cse4 turnover as Cse4 degradation is compromised in yeast cells lacking RCY1. Excessive Cse4 accumulation in rcy1β cells leads to growth retardation. Furthermore, the deletion of RCY1 is tied to enhanced chromosome instability and temperature- sensitive cell growth. Our results reveal the involvement of Rcy1 in chromosome regulation and another regulatory pathway controlling the Cse4 level and activity.

Original languageEnglish (US)
Pages (from-to)10372-10377
Number of pages6
JournalJournal of Biological Chemistry
Volume291
Issue number19
DOIs
StatePublished - May 6 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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