TY - JOUR
T1 - The F-box protein rcy1 is involved in the degradation of histone H3 variant Cse4 and genome maintenance
AU - Cheng, Haili
AU - Bao, Xin
AU - Rao, Hai
N1 - Funding Information:
This work was supported by Welch Foundation Grant AQ 1747, the William & Ella Owens Medical Research Foundation, the Helen F. Kerr Foundation, Department of Defense Grant W911NF-11-10466, and National Institutes of Health Grant GM 078085 (to H. R.).
PY - 2016/5/6
Y1 - 2016/5/6
N2 - Cse4, a histone H3-like centromeric protein, plays critical functions in chromosome segregation. Cse4 level is tightly regulated, but the underlying mechanism remains poorly understood. We employed a toxicity-based screen to look for the degradation components involved in Cse4 regulation. Here, we show that the F-box containing protein Rcy1 is required for efficient Cse4 turnover as Cse4 degradation is compromised in yeast cells lacking RCY1. Excessive Cse4 accumulation in rcy1β cells leads to growth retardation. Furthermore, the deletion of RCY1 is tied to enhanced chromosome instability and temperature- sensitive cell growth. Our results reveal the involvement of Rcy1 in chromosome regulation and another regulatory pathway controlling the Cse4 level and activity.
AB - Cse4, a histone H3-like centromeric protein, plays critical functions in chromosome segregation. Cse4 level is tightly regulated, but the underlying mechanism remains poorly understood. We employed a toxicity-based screen to look for the degradation components involved in Cse4 regulation. Here, we show that the F-box containing protein Rcy1 is required for efficient Cse4 turnover as Cse4 degradation is compromised in yeast cells lacking RCY1. Excessive Cse4 accumulation in rcy1β cells leads to growth retardation. Furthermore, the deletion of RCY1 is tied to enhanced chromosome instability and temperature- sensitive cell growth. Our results reveal the involvement of Rcy1 in chromosome regulation and another regulatory pathway controlling the Cse4 level and activity.
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U2 - 10.1074/jbc.M115.701813
DO - 10.1074/jbc.M115.701813
M3 - Article
C2 - 26975376
AN - SCOPUS:84966269225
VL - 291
SP - 10372
EP - 10377
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 19
ER -