The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum

David D. Dean, A. J. Domnas

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalExperimental Mycology
Volume7
Issue number1
DOIs
StatePublished - Mar 1983

Keywords

  • Lagenidium giganteum
  • collagenase
  • extracellular proteases
  • pathogenesis in mosquito larvae
  • trypsin-like

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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