Abstract
Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31-39 |
| Number of pages | 9 |
| Journal | Experimental Mycology |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1983 |
Keywords
- Lagenidium giganteum
- collagenase
- extracellular proteases
- pathogenesis in mosquito larvae
- trypsin-like
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
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Dean, D. D., & Domnas, A. J. (1983). The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum. Experimental Mycology, 7(1), 31-39. https://doi.org/10.1016/0147-5975(83)90072-5