The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum

David D. Dean; A. J. Domnas

doi:10.1016/0147-5975(83)90072-5

The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum

David D. Dean, A. J. Domnas

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.

Original language	English (US)
Pages (from-to)	31-39
Number of pages	9
Journal	Experimental Mycology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/0147-5975(83)90072-5
State	Published - Mar 1983
Externally published	Yes

Keywords

Lagenidium giganteum
collagenase
extracellular proteases
pathogenesis in mosquito larvae
trypsin-like

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1016/0147-5975(83)90072-5

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title = "The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum",

abstract = "Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.",

keywords = "Lagenidium giganteum, collagenase, extracellular proteases, pathogenesis in mosquito larvae, trypsin-like",

author = "Dean, {David D.} and Domnas, {A. J.}",

note = "Funding Information: This paper represents a portion of a Ph.D. dissertation presented by D. D. Dean to the Department of Botany at the University of North Carolina at Chapel Hill. The work was supported, in part, by a grant-in-aid of research from the Society of Sigma Xi (D. D. Dean), the UNC Smith Fund (D. D. Dean), an NIH Biomedical Fellowship (D. D. Dean), and NIH Grant 1-ROl-AI17024-01 (A. J. Domnas). The authors would like to thank Dr. J. D. Geratz for a reading of the manuscript and the gift of benzamidine, pentamidine, and I-RT-63, Dr. J. Harrison for a reading of the manuscript, and Ms. Susan Sizemore for her technical help in the preparation of figures and graphs.",

year = "1983",

month = mar,

doi = "10.1016/0147-5975(83)90072-5",

language = "English (US)",

volume = "7",

pages = "31--39",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Academic Press Inc.",

number = "1",

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AU - Dean, David D.

AU - Domnas, A. J.

N1 - Funding Information: This paper represents a portion of a Ph.D. dissertation presented by D. D. Dean to the Department of Botany at the University of North Carolina at Chapel Hill. The work was supported, in part, by a grant-in-aid of research from the Society of Sigma Xi (D. D. Dean), the UNC Smith Fund (D. D. Dean), an NIH Biomedical Fellowship (D. D. Dean), and NIH Grant 1-ROl-AI17024-01 (A. J. Domnas). The authors would like to thank Dr. J. D. Geratz for a reading of the manuscript and the gift of benzamidine, pentamidine, and I-RT-63, Dr. J. Harrison for a reading of the manuscript, and Ms. Susan Sizemore for her technical help in the preparation of figures and graphs.

PY - 1983/3

Y1 - 1983/3

N2 - Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.

AB - Lagenidium giganteum produces extracellular proteases when grown in peptone-yeast extract-glucose medium. The production of the enzymes required a suitable inducer such as protein, and was repressed by glucose. The total filtrate proteases had a pH optimum of 8.4, and were heat denatured t 60°C. The use of specific substrates and inhibitors demonstrated the presence of collagenase, trypsin-like protease, and a weak elastase. Trypsin-like activity was present in the medium after 38 h of growth, whereas collagenase appeared in 43 h. The roles of these enzymes in pathogenesis were discussed.

KW - Lagenidium giganteum

KW - collagenase

KW - extracellular proteases

KW - pathogenesis in mosquito larvae

KW - trypsin-like

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The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum

Abstract

Keywords

ASJC Scopus subject areas

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