The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age

Nian Shang Cai; Ding Dong Li; H. Tak Cheung; Arlan Richardson

doi:10.1016/0008-8749(90)90274-U

The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age

Nian Shang Cai, Ding Dong Li, H. Tak Cheung, Arlan Richardson

Barshop Institute

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.

Original language	English (US)
Pages (from-to)	311-319
Number of pages	9
Journal	Cellular Immunology
Volume	130
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(90)90274-U
State	Published - Oct 15 1990

ASJC Scopus subject areas

Immunology

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@article{c8515f9dc1ca451b9a48d391c0b019ec,

title = "The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age",

abstract = "The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.",

author = "Cai, {Nian Shang} and Li, {Ding Dong} and Cheung, {H. Tak} and Arlan Richardson",

note = "Funding Information: This work was supported by Grants AG 0 1548 and AG 00390 from the National Institutes of Health. We thank the following investigators for generously supplying the cDNA probes: mouse IL-2 cDNA probe from Dr. Tony Burgess (Institute for Cancer Research, Melbourn, Australia), and mouse IL-3 and GM-CSF cDNA probes from Dr. K. Arai (DNAX Research Institute, Palo Alto, CA).",

year = "1990",

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doi = "10.1016/0008-8749(90)90274-U",

language = "English (US)",

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journal = "Cellular Immunology",

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T1 - The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age

AU - Cai, Nian Shang

AU - Li, Ding Dong

AU - Cheung, H. Tak

AU - Richardson, Arlan

N1 - Funding Information: This work was supported by Grants AG 0 1548 and AG 00390 from the National Institutes of Health. We thank the following investigators for generously supplying the cDNA probes: mouse IL-2 cDNA probe from Dr. Tony Burgess (Institute for Cancer Research, Melbourn, Australia), and mouse IL-3 and GM-CSF cDNA probes from Dr. K. Arai (DNAX Research Institute, Palo Alto, CA).

PY - 1990/10/15

Y1 - 1990/10/15

N2 - The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.

AB - The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.

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