Abstract
The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.
Original language | English (US) |
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Pages (from-to) | 311-319 |
Number of pages | 9 |
Journal | Cellular Immunology |
Volume | 130 |
Issue number | 2 |
DOIs | |
State | Published - Oct 15 1990 |
ASJC Scopus subject areas
- Immunology