The establishment of a fast and safe orthotopic colon cancer model using a tissue adhesive technique

Hong Tao Hu, Zhe Wang, Myung Ji Kim, Lu Shang Jiang, Shi Jun Xu, Jaeyun Jung, Eunji Lee, Jung Hoon Park, Nader Bakheet, Sung Hwan Yoon, Kun Yung Kim, Ho Young Song, Suhwan Chang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Purpose We aimed to develop a novel method for orthotopic colon cancer model, using tissue adhesive in place of conventional surgical method. Materials and Methods RFP HCT 116 cell line were used to establish the colon cancer model. Fresh tumor tissue harvested from a subcutaneous injection was grafted into twenty nude mice, divided into group A (suture method) and group B (tissue adhesive method). For the group A, we fixed the tissue on the serosa layer of proximal colon by 8-0 surgical suture. For the group B, tissue adhesive (10 μL) was used to fix the tumor. The mortality, tumor implantation success, tumor metastasis, primary tumor size, and operation time were compared between the two groups. Dissected tumor tissue was analyzed for the histology and immunohistochemistry. Also, we performed tumor marker analysis. Results We observed 30% increase in graft success and 20% decrease in mortality, by using tissue adhesive method, respectively. The median colon tumor size was significantly increased by 4 mm and operation time was shortened by 6.5 minutes. The H&E showed similar tumor structure between the two groups. The immunohistochemistry staining for cancer antigen 19-9, carcinoembryonic antigen, cytokeratin 20, and Ki-67 showed comparable intensities in both groups. Real-time quantitative reverse transcription analysis showed eight out of nine tumor markers are unchanged in the tissue adhesive group. Western blot indicated the tissue adhesive group expressed less p-JNK (apototic marker) and more p-MEK/p-p38 (proliferation marker) levels. Conclusion We concluded the tissue adhesive method is a quick and safe way to generate orthotopic, colon cancer model.

Original languageEnglish (US)
Pages (from-to)733-743
Number of pages11
JournalCancer Research and Treatment
Volume53
Issue number3
DOIs
StatePublished - 2021
Externally publishedYes

Keywords

  • Colon neoplasms
  • Mouse model
  • Orthotopic
  • Tissue adhesive technique

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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