The effects of Tween 80, Myrj 52 and PEG 8000 upon the rate of pentobarbital disappearance, cumulative water flux and histological changes in the rat small intestine

Walter G. Gowan, Fermin Tio, Kenneth Lamp, Christie Gowan, S. Stavchansky

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The effect of aqueous solutions of 10-2, 10-3, 10-4 and 10-6 M concentrations of Tween 80, Myrj 52 and PEG 8000 on the cumulative water flux and rate of disappearance of pentobarbital from the rat small intestine was investigated using the in situ rat gut technique. Water served as the control treatment. Tissue samples of the small intestine were examined microscopically to determine if the histological effects of different solutions (10 -2 and 10 -4 M) could be related to the disappearance rate and cumulative water flux data. An inverse relationship between rate of disappearance and concentration was observed. The same relationship was also noted with cumulative water flux and concentration. A general relationship between high rate of disappearance and a high cumulative water flux was also noted. The Tween 80 solutions exhibited a high water flux at all concentrations. The microscopic examination of the tissues revealed that those solutions which caused the most significant disturbances in the mucous layer and the interstitial spaces resulted in the lowest rates and fluxes. The relative order of the differences noted in the histological evaluation were directly related to the differences noted in the rates and fluxes of the different treatments. The Tween 80 solutions had the least effect on the mucous layer and the amount of water in the interstitial spaces. These solutions also exhibited statistically greater (P < 0.05) values for the cumulative water flux and rate of drug disappearance from the lumen when compared to the other treatments or to the water controls. The 10-2 M solutions of Myrj 52 and PEG 8000 demonstrated significant microscopic changes and low values for the cumulative water flux and rate of pentobarbital disappearance. The Tween 80 solutions demonstrated flux, rate and histological changes that were inconsistent with the data observed in the Myrj 52 and PEG 8000 solutions.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume29
Issue number2-3
DOIs
StatePublished - Apr 1986
Externally publishedYes

Keywords

  • in situ drug absorption
  • Myrj 52
  • PEG 8000
  • rate of drug disappearance
  • Tween 80
  • water flux

ASJC Scopus subject areas

  • Pharmaceutical Science

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