THE EFFECTS OF LOW DENSITY LIPOPROTEINS ON UPTAKE OF PHOTOFRIN II

Mladen Korbelik, Jaclyn Hung, Stephen Lam, Branko Palcic

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Abstract— The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT‐F tumor implanted in mice. Results showed that serum inhibited PII uptake. In general, LDL also inhibits PII uptake with the exception of an initial increase in the first10–30 min when the cellular concentration of PII was measured by fluorescence imaging instead of chemical extraction. Our results suggest a possible de‐aggregation process occurring upon internalization or binding of PII to LDL.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalPhotochemistry and Photobiology
Volume51
Issue number2
DOIs
StatePublished - Feb 1990

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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