Abstract
The involvement of acidic cellular compartments that function in biosynthetic roles has recently become an important facet in understanding the regulation of glycoconjugate biosynthesis. Treating cells with increasing concentrations of ammonium chloride (1-50 mM) resulted in about a 50% increase in the synthesis and secretion of newly synthesized [3H]glucosamine-labeled and 35SO4-labeled endothelial glycoproteins. In contrast, these increasing concentrations of ammonium chloride resulted in a marked decrease in the synthesis of [3H]glucosamine- and 35SO4-labeled secreted proteoglycans. Thus the apparent neutralization of acidic compartments does not adversely effect glycoprotein biosynthesis.
Original language | English (US) |
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Pages (from-to) | 1022-1023 |
Number of pages | 2 |
Journal | IRCS Medical Science |
Volume | 11 |
Issue number | 11 |
State | Published - 1983 |
Externally published | Yes |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology