The involvement of acidic cellular compartments that function in biosynthetic roles has recently become an important facet in understanding the regulation of glycoconjugate biosynthesis. Treating cells with increasing concentrations of ammonium chloride (1-50 mM) resulted in about a 50% increase in the synthesis and secretion of newly synthesized [3H]glucosamine-labeled and 35SO4-labeled endothelial glycoproteins. In contrast, these increasing concentrations of ammonium chloride resulted in a marked decrease in the synthesis of [3H]glucosamine- and 35SO4-labeled secreted proteoglycans. Thus the apparent neutralization of acidic compartments does not adversely effect glycoprotein biosynthesis.
|Original language||English (US)|
|Number of pages||2|
|Journal||IRCS Medical Science|
|State||Published - Jan 1 1983|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)