TY - JOUR
T1 - The effect of hydration stress solutes on the phase behavior of hydrated dipalmitoylphosphatidylcholine
AU - Rudolph, Alan S.
AU - Goins, Beth
N1 - Funding Information:
The authors would like to thank Mr. Richard Cliff for his technical assistance with the photon correlation spectrometer. Many helpful discussions with Dr. Gordon Jendrasiak are also acknowledged. The authors would also like to gratefully acknowledge financial support for this work through the Naval Medical Research and Development Command, Accelerated Research Initiative at the Naval Research Laboratory, and the National Research Council of which Dr. Goins is a post-doctoral fellow.
PY - 1991/7/1
Y1 - 1991/7/1
N2 - We have investigated the interaction of solutes found to accumulate in biological systems during chilling, dehydration, and salt stress with fully hydrated multilamellar and unilamellar vesicles of dipalmitoylphosphatidylcholine (DPPC). We have focused on a series of mono-, di-, and tri-substituted amines (glycine, 4-hydroxyproline, proline, and betaine) and contrasted the action of these solutes to trehalose, a protective disaccharide. Differential scanning calorimetry studies show that when DPPC is scanned in the presence of increasing concentrations of these solutes (up to 3 M), there is a moderate increase in the pre-transition temperature (1-6°C) with a smaller increase (1-2°C) in the main transition temperature of hydrated multilamellar vesicles of DPPC. Other calorimetric parameters (ΔH, ΔT 1 2, Cpmax) determined for the pre-transition and main transition were similar independent of the solute. In each case, the main phase transition was broadened with increasing solute while the transition enthalpy was not significantly affected.
AB - We have investigated the interaction of solutes found to accumulate in biological systems during chilling, dehydration, and salt stress with fully hydrated multilamellar and unilamellar vesicles of dipalmitoylphosphatidylcholine (DPPC). We have focused on a series of mono-, di-, and tri-substituted amines (glycine, 4-hydroxyproline, proline, and betaine) and contrasted the action of these solutes to trehalose, a protective disaccharide. Differential scanning calorimetry studies show that when DPPC is scanned in the presence of increasing concentrations of these solutes (up to 3 M), there is a moderate increase in the pre-transition temperature (1-6°C) with a smaller increase (1-2°C) in the main transition temperature of hydrated multilamellar vesicles of DPPC. Other calorimetric parameters (ΔH, ΔT 1 2, Cpmax) determined for the pre-transition and main transition were similar independent of the solute. In each case, the main phase transition was broadened with increasing solute while the transition enthalpy was not significantly affected.
KW - Dipalmitoylphosphatidylcholine
KW - Hydration
KW - Phase behavior
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U2 - 10.1016/0005-2736(91)90255-7
DO - 10.1016/0005-2736(91)90255-7
M3 - Article
C2 - 2065073
AN - SCOPUS:0025891485
VL - 1066
SP - 90
EP - 94
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -