The effect of gonadectomy and estradiol on sensitivity to oxidative stress

Alex F. Bokov, Daijin Ko, Arlan Richardson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The sexual dimorphism of life span and caloric restriction effects in numerous species suggest that estradiol (E2) is protective against oxidative damage. The only direct test of E2's protective effect in mice against in vivo oxidative stress to date may have been confounded by E2's direct chemical action as an antioxidant because it was administered at very high dosages. Therefore, we have identified a low yet physiologically effective dose ofE2. We then administered this dose using subcutaneous time-release pellets to ovariectomized mice. Two weeks after E2 pellet implantation, sham-operated, ovariectomized, and ovariectomized E2-supplemented female mice were injected with a lethal dose of paraquat and their survival was followed. It was observed that ovariectomy exacerbates paraquat-induced mortality and is rescued by E2 supplementation. An equivalent experiment was performed on sham-operated, orchidectomized, and E2-supplemenled orchidectomized male mice. The survival of male mice was improved by orchidectomy, and E2 gave no further benefit. We interpret the results to mean that E2 is protective against oxidative stress through its regulatory role and that testosterone diminishes protection against oxidative stress.

Original languageEnglish (US)
Pages (from-to)43-58
Number of pages16
JournalEndocrine Research
Volume34
Issue number1-2
DOIs
StatePublished - Mar 2009

Fingerprint

Estradiol
Oxidative Stress
Paraquat
Pharmacologic Actions
Caloric Restriction
Orchiectomy
Ovariectomy
Sex Characteristics
Testosterone
Antioxidants
Mortality

Keywords

  • Estradiol
  • Gonadectomy
  • Mice
  • Oxidative stress
  • Paraquat

ASJC Scopus subject areas

  • Endocrinology

Cite this

The effect of gonadectomy and estradiol on sensitivity to oxidative stress. / Bokov, Alex F.; Ko, Daijin; Richardson, Arlan.

In: Endocrine Research, Vol. 34, No. 1-2, 03.2009, p. 43-58.

Research output: Contribution to journalArticle

Bokov, Alex F. ; Ko, Daijin ; Richardson, Arlan. / The effect of gonadectomy and estradiol on sensitivity to oxidative stress. In: Endocrine Research. 2009 ; Vol. 34, No. 1-2. pp. 43-58.
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