The effect of glass-ceramic implants on matrix vesicle calcification after two weeks of rat tibial bone healing

C. Muller-Mai, D. Amir, H. Wendland, Zvi Schwartz, J. Sela, U. Gross

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Type, size and distribution of extracellular matrix vesicles (MV), known mediators of primary calcification, were studied around bone-bonding and metal-oxide containing, nonbonding, glass-ceramic implants. This was performed in order to further understand the different effects of implants on bone healing. At 14 days after implantation in adult rat tibial bone the effects of different implants on MV were studied by transmission electron microscopy and computerized morphometry. A total number of 4607 MV in 245 electron micrographs were counted and grouped according to diameter, distance from the calcifying front, and classified as four types: 'empty', 'amorphous,' 'crystal', and 'rupture'. The sequence of types according to diameter and distance was recorded as follows around both implants tested: 'rupture' MV were the closet to the front with the largest diameter, followed by 'crystal,' 'amorphous,' and 'empty,' MV with the largest distance from the front and the smallest diameter. Most vesicles were concentrated in a distance of less than 2.4 μm from the front and between diameters of 0.06 μm and 0.22 μm. The noncalcified extracellular matrix around bone-bonding implants contained more MV than the matrix around ' '

Original languageEnglish (US)
Pages (from-to)1571-1584
Number of pages14
JournalJournal of Biomedical Materials Research
Volume24
Issue number12
DOIs
StatePublished - Dec 1990
Externally publishedYes

Fingerprint

Glass ceramics
Rats
Bone
Bone and Bones
Extracellular Matrix
Rupture
Crystals
Transmission Electron Microscopy
Oxides
Metals
Electrons
Transmission electron microscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

The effect of glass-ceramic implants on matrix vesicle calcification after two weeks of rat tibial bone healing. / Muller-Mai, C.; Amir, D.; Wendland, H.; Schwartz, Zvi; Sela, J.; Gross, U.

In: Journal of Biomedical Materials Research, Vol. 24, No. 12, 12.1990, p. 1571-1584.

Research output: Contribution to journalArticle

Muller-Mai, C. ; Amir, D. ; Wendland, H. ; Schwartz, Zvi ; Sela, J. ; Gross, U. / The effect of glass-ceramic implants on matrix vesicle calcification after two weeks of rat tibial bone healing. In: Journal of Biomedical Materials Research. 1990 ; Vol. 24, No. 12. pp. 1571-1584.
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