Abstract
The size of the poly(A) segment of poly(A)+RNA extracted from hepatocytes isolated from 4- to 30-month-old rats was determined using polyacrylamide-agarose gel electrophoresis. The average size of the poly(A) segment isolated from newly synthesized poly(A)+RNA was 150 nucleotides, and the average size of the poly(A) segment isolated from the steady state pool of cytoplasmic poly(A)+RNA was 70 nucleotides. No significant age related change in either the size or the heterogeneity of the newly synthesized poly(A) segment was found. The translational activity of poly(A)+RNA isolated from livers of 4- to 30-month-old rats was determined using wheat germ extract and rabbit reticulocyte lysate cell-free systems. No age-related change in the translational activity of the poly(A)+RNA was observed. The effect of aging on the cap structure was also studied by measuring the inhibition of cell-free translation by 7-methyl guanosine-5′-monophosphate. No age-related change in the sensitivity of the poly(A)+RNA preparations to inhibition of translation by 7-methyl guanosine-5′-monophosphate was observed. Therefore, the results of this study demonstrate that no major age-related change occurs in the structure of the poly(A) segment, the translational activity, or the cap structure of poly(A)+RNA from rat liver.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 99-111 |
| Number of pages | 13 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 32 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 14 1985 |
| Externally published | Yes |
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Keywords
- Cap structure
- Liver
- Messenger RNA
- Poly(A) segment
- Protein synthesis
- Translation
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
The effect of aging on the structure and function of liver messenger RNA. / Birchenall-Sparks, M. C.; Roberts, M. S.; Rutherford, M. S.; Richardson, A.
In: Mechanisms of Ageing and Development, Vol. 32, No. 1, 14.10.1985, p. 99-111.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of aging on the structure and function of liver messenger RNA
AU - Birchenall-Sparks, M. C.
AU - Roberts, M. S.
AU - Rutherford, M. S.
AU - Richardson, A.
PY - 1985/10/14
Y1 - 1985/10/14
N2 - The size of the poly(A) segment of poly(A)+RNA extracted from hepatocytes isolated from 4- to 30-month-old rats was determined using polyacrylamide-agarose gel electrophoresis. The average size of the poly(A) segment isolated from newly synthesized poly(A)+RNA was 150 nucleotides, and the average size of the poly(A) segment isolated from the steady state pool of cytoplasmic poly(A)+RNA was 70 nucleotides. No significant age related change in either the size or the heterogeneity of the newly synthesized poly(A) segment was found. The translational activity of poly(A)+RNA isolated from livers of 4- to 30-month-old rats was determined using wheat germ extract and rabbit reticulocyte lysate cell-free systems. No age-related change in the translational activity of the poly(A)+RNA was observed. The effect of aging on the cap structure was also studied by measuring the inhibition of cell-free translation by 7-methyl guanosine-5′-monophosphate. No age-related change in the sensitivity of the poly(A)+RNA preparations to inhibition of translation by 7-methyl guanosine-5′-monophosphate was observed. Therefore, the results of this study demonstrate that no major age-related change occurs in the structure of the poly(A) segment, the translational activity, or the cap structure of poly(A)+RNA from rat liver.
AB - The size of the poly(A) segment of poly(A)+RNA extracted from hepatocytes isolated from 4- to 30-month-old rats was determined using polyacrylamide-agarose gel electrophoresis. The average size of the poly(A) segment isolated from newly synthesized poly(A)+RNA was 150 nucleotides, and the average size of the poly(A) segment isolated from the steady state pool of cytoplasmic poly(A)+RNA was 70 nucleotides. No significant age related change in either the size or the heterogeneity of the newly synthesized poly(A) segment was found. The translational activity of poly(A)+RNA isolated from livers of 4- to 30-month-old rats was determined using wheat germ extract and rabbit reticulocyte lysate cell-free systems. No age-related change in the translational activity of the poly(A)+RNA was observed. The effect of aging on the cap structure was also studied by measuring the inhibition of cell-free translation by 7-methyl guanosine-5′-monophosphate. No age-related change in the sensitivity of the poly(A)+RNA preparations to inhibition of translation by 7-methyl guanosine-5′-monophosphate was observed. Therefore, the results of this study demonstrate that no major age-related change occurs in the structure of the poly(A) segment, the translational activity, or the cap structure of poly(A)+RNA from rat liver.
KW - Cap structure
KW - Liver
KW - Messenger RNA
KW - Poly(A) segment
KW - Protein synthesis
KW - Translation
UR - http://www.scopus.com/inward/record.url?scp=0022373882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022373882&partnerID=8YFLogxK
U2 - 10.1016/0047-6374(85)90039-9
DO - 10.1016/0047-6374(85)90039-9
M3 - Article
C2 - 2423817
AN - SCOPUS:0022373882
VL - 32
SP - 99
EP - 111
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 1
ER -