The effect of aging on the structure and function of liver messenger RNA

M. C. Birchenall-Sparks; M. S. Roberts; M. S. Rutherford; A. Richardson

doi:10.1016/0047-6374(85)90039-9

The effect of aging on the structure and function of liver messenger RNA

M. C. Birchenall-Sparks, M. S. Roberts, M. S. Rutherford, A. Richardson

Barshop Institute

Research output: Contribution to journal › Article

12 Scopus citations

Abstract

The size of the poly(A) segment of poly(A)⁺RNA extracted from hepatocytes isolated from 4- to 30-month-old rats was determined using polyacrylamide-agarose gel electrophoresis. The average size of the poly(A) segment isolated from newly synthesized poly(A)⁺RNA was 150 nucleotides, and the average size of the poly(A) segment isolated from the steady state pool of cytoplasmic poly(A)⁺RNA was 70 nucleotides. No significant age related change in either the size or the heterogeneity of the newly synthesized poly(A) segment was found. The translational activity of poly(A)⁺RNA isolated from livers of 4- to 30-month-old rats was determined using wheat germ extract and rabbit reticulocyte lysate cell-free systems. No age-related change in the translational activity of the poly(A)⁺RNA was observed. The effect of aging on the cap structure was also studied by measuring the inhibition of cell-free translation by 7-methyl guanosine-5′-monophosphate. No age-related change in the sensitivity of the poly(A)⁺RNA preparations to inhibition of translation by 7-methyl guanosine-5′-monophosphate was observed. Therefore, the results of this study demonstrate that no major age-related change occurs in the structure of the poly(A) segment, the translational activity, or the cap structure of poly(A)⁺RNA from rat liver.

Original language	English (US)
Pages (from-to)	99-111
Number of pages	13
Journal	Mechanisms of Ageing and Development
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0047-6374(85)90039-9
State	Published - Oct 14 1985

Keywords

Cap structure
Liver
Messenger RNA
Poly(A) segment
Protein synthesis
Translation

ASJC Scopus subject areas

Aging
Developmental Biology

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Birchenall-Sparks, M. C., Roberts, M. S., Rutherford, M. S., & Richardson, A. (1985). The effect of aging on the structure and function of liver messenger RNA. Mechanisms of Ageing and Development, 32(1), 99-111. https://doi.org/10.1016/0047-6374(85)90039-9

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