The effect of aging on the expression of interleukin 2 messenger ribonucleic acid

Wutong Wu; Mohammad Pahlavani; H. Tak Cheung; Arlan Richardson

doi:10.1016/0008-8749(86)90021-3

The effect of aging on the expression of interleukin 2 messenger ribonucleic acid

Wutong Wu, Mohammad Pahlavani, H. Tak Cheung, Arlan Richardson

Barshop Institute

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

It is well documented that the production of interleukin 2 (IL-2) by lymphocytes declines with increasing age. Using a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes. The induction of IL-2 mRNA by concanavalin A was maximum after 20 hr of incubation. Northern blots demonstrated that the cDNA probe to IL-2 hybridized to an 11 S to 12 S RNA species. No age-related change in the size of the IL-2 mRNA was observed. In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA. The induction of IL-2 mRNA decreased 85% between 5 and 29 months and paralleled the decline in IL-2 production and lymphocyte proliferation. Therefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.

Original language	English (US)
Pages (from-to)	224-231
Number of pages	8
Journal	Cellular Immunology
Volume	100
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(86)90021-3
State	Published - Jun 1986

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(86)90021-3

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title = "The effect of aging on the expression of interleukin 2 messenger ribonucleic acid",

abstract = "It is well documented that the production of interleukin 2 (IL-2) by lymphocytes declines with increasing age. Using a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes. The induction of IL-2 mRNA by concanavalin A was maximum after 20 hr of incubation. Northern blots demonstrated that the cDNA probe to IL-2 hybridized to an 11 S to 12 S RNA species. No age-related change in the size of the IL-2 mRNA was observed. In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA. The induction of IL-2 mRNA decreased 85% between 5 and 29 months and paralleled the decline in IL-2 production and lymphocyte proliferation. Therefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.",

author = "Wutong Wu and Mohammad Pahlavani and Cheung, {H. Tak} and Arlan Richardson",

note = "Funding Information: {\textquoteright} This work was supported by Grant AG 04520 from the National Institute of Aging, Bethesda, Md. * Permanent address:D epartment of Biochemistry, Nanjing College of Pharmacy, Nanjing, The People{\textquoteright}s Republic of China. 3 To whom correspondence should be addressed.",

year = "1986",

month = jun,

doi = "10.1016/0008-8749(86)90021-3",

language = "English (US)",

volume = "100",

pages = "224--231",

journal = "Cellular Immunology",

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T1 - The effect of aging on the expression of interleukin 2 messenger ribonucleic acid

AU - Wu, Wutong

AU - Pahlavani, Mohammad

AU - Cheung, H. Tak

AU - Richardson, Arlan

N1 - Funding Information: ’ This work was supported by Grant AG 04520 from the National Institute of Aging, Bethesda, Md. * Permanent address:D epartment of Biochemistry, Nanjing College of Pharmacy, Nanjing, The People’s Republic of China. 3 To whom correspondence should be addressed.

PY - 1986/6

Y1 - 1986/6

N2 - It is well documented that the production of interleukin 2 (IL-2) by lymphocytes declines with increasing age. Using a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes. The induction of IL-2 mRNA by concanavalin A was maximum after 20 hr of incubation. Northern blots demonstrated that the cDNA probe to IL-2 hybridized to an 11 S to 12 S RNA species. No age-related change in the size of the IL-2 mRNA was observed. In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA. The induction of IL-2 mRNA decreased 85% between 5 and 29 months and paralleled the decline in IL-2 production and lymphocyte proliferation. Therefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.

AB - It is well documented that the production of interleukin 2 (IL-2) by lymphocytes declines with increasing age. Using a cDNA probe to IL-2, the effect of age on the expression of IL-2 mRNA was studied on concanavalin A-stimulated lymphocytes. The induction of IL-2 mRNA by concanavalin A was maximum after 20 hr of incubation. Northern blots demonstrated that the cDNA probe to IL-2 hybridized to an 11 S to 12 S RNA species. No age-related change in the size of the IL-2 mRNA was observed. In addition, there was no evidence of an age-related change in IL-2 mRNA degradation or the post-transcriptional processing of IL-2 mRNA. The induction of IL-2 mRNA decreased 85% between 5 and 29 months and paralleled the decline in IL-2 production and lymphocyte proliferation. Therefore, the age-related decline in the ability of lymphocyte population to produce IL-2 arises from a decrease in the ability of the lymphocytes to genetically express IL-2 mRNA.

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JO - Cellular Immunology

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The effect of aging on the expression of interleukin 2 messenger ribonucleic acid

Abstract

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