The effect of aging and dietary restriction on DNA repair

Nataya Weraarchakul, John R Strong, W. Gibson Wood, Arlan Richardson

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

DNA repair was studied as a function of age in cells isolated from both the liver and the kidney of male Fischer F344 rats. DNA repair was measured by quantifying unscheduled DNA synthesis induced by UV irradiation. Unscheduled DNA synthesis decreased approximately 50% between the ages of 5 and 30 months in both hepatocytes and kidney cells. The age-related decline in unscheduled DNA synthesis in cells isolated from the liver and kidney was compared in rats fed ad libitum and rats fed a calorie-restricted diet; calorie restriction has been shown to increase the survival of rodents. The level of unscheduled DNA synthesis was significantly higher in hepatocytes and kidney cells isolated from the rats fed the restricted diet. Thus, calorie restriction appears to retard the age-related decline in DNA repair.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalExperimental Cell Research
Volume181
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

DNA Repair
Kidney
DNA
Inbred F344 Rats
Hepatocytes
Diet
Liver
Rodentia

ASJC Scopus subject areas

  • Cell Biology

Cite this

The effect of aging and dietary restriction on DNA repair. / Weraarchakul, Nataya; Strong, John R; Wood, W. Gibson; Richardson, Arlan.

In: Experimental Cell Research, Vol. 181, No. 1, 1989, p. 197-204.

Research output: Contribution to journalArticle

Weraarchakul, Nataya ; Strong, John R ; Wood, W. Gibson ; Richardson, Arlan. / The effect of aging and dietary restriction on DNA repair. In: Experimental Cell Research. 1989 ; Vol. 181, No. 1. pp. 197-204.
@article{f568279004ed44f5a218eb341ce7d9af,
title = "The effect of aging and dietary restriction on DNA repair",
abstract = "DNA repair was studied as a function of age in cells isolated from both the liver and the kidney of male Fischer F344 rats. DNA repair was measured by quantifying unscheduled DNA synthesis induced by UV irradiation. Unscheduled DNA synthesis decreased approximately 50{\%} between the ages of 5 and 30 months in both hepatocytes and kidney cells. The age-related decline in unscheduled DNA synthesis in cells isolated from the liver and kidney was compared in rats fed ad libitum and rats fed a calorie-restricted diet; calorie restriction has been shown to increase the survival of rodents. The level of unscheduled DNA synthesis was significantly higher in hepatocytes and kidney cells isolated from the rats fed the restricted diet. Thus, calorie restriction appears to retard the age-related decline in DNA repair.",
author = "Nataya Weraarchakul and Strong, {John R} and Wood, {W. Gibson} and Arlan Richardson",
year = "1989",
doi = "10.1016/0014-4827(89)90193-6",
language = "English (US)",
volume = "181",
pages = "197--204",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The effect of aging and dietary restriction on DNA repair

AU - Weraarchakul, Nataya

AU - Strong, John R

AU - Wood, W. Gibson

AU - Richardson, Arlan

PY - 1989

Y1 - 1989

N2 - DNA repair was studied as a function of age in cells isolated from both the liver and the kidney of male Fischer F344 rats. DNA repair was measured by quantifying unscheduled DNA synthesis induced by UV irradiation. Unscheduled DNA synthesis decreased approximately 50% between the ages of 5 and 30 months in both hepatocytes and kidney cells. The age-related decline in unscheduled DNA synthesis in cells isolated from the liver and kidney was compared in rats fed ad libitum and rats fed a calorie-restricted diet; calorie restriction has been shown to increase the survival of rodents. The level of unscheduled DNA synthesis was significantly higher in hepatocytes and kidney cells isolated from the rats fed the restricted diet. Thus, calorie restriction appears to retard the age-related decline in DNA repair.

AB - DNA repair was studied as a function of age in cells isolated from both the liver and the kidney of male Fischer F344 rats. DNA repair was measured by quantifying unscheduled DNA synthesis induced by UV irradiation. Unscheduled DNA synthesis decreased approximately 50% between the ages of 5 and 30 months in both hepatocytes and kidney cells. The age-related decline in unscheduled DNA synthesis in cells isolated from the liver and kidney was compared in rats fed ad libitum and rats fed a calorie-restricted diet; calorie restriction has been shown to increase the survival of rodents. The level of unscheduled DNA synthesis was significantly higher in hepatocytes and kidney cells isolated from the rats fed the restricted diet. Thus, calorie restriction appears to retard the age-related decline in DNA repair.

UR - http://www.scopus.com/inward/record.url?scp=0024544283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024544283&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(89)90193-6

DO - 10.1016/0014-4827(89)90193-6

M3 - Article

C2 - 2917602

AN - SCOPUS:0024544283

VL - 181

SP - 197

EP - 204

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -