The effect of age on the synthesis of two heat shock proteins in the HSP70 family

B. Wu, M. J. Gu, A. R. Heydari, A. Richardson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Hepatocytes isolated from male F344 rats were exposed to elevated temperatures (40 °C to 45 °C), and the incorporation of [35S]-L- methionine into proteins was measured from fluorograms of two-dimensional polyacrylamide gels. The synthesis of two proteins was induced by temperatures of 40 °C to 42.5 °C; however, 45 °C inhibited the synthesis of all proteins. Based on their apparent molecular weights and pI values and their recognition by a monoclonal antibody to the HSP70 gene family, the two proteins induced by hyperthermia were found to be the highly heat-inducible hsp70 and the constitutive hsc70. Because a heat shock of 42.5 °C for 30 minutes had very little effect on cell viability and induced the synthesis of hsp70 and hsc70, this heat shock was used to study the effect of age on the ability of hepatocytes to respond to the stress of hyperthermia. Neither hepatocytes isolated from young adult (5-7 months) nor old (25-27 months) rats synthesized detectable amounts of hsp70 when incubated at 37 °C. However, heat shock induced the synthesis of both hsp70 and hsc70 in hepatocytes isolated from young adult and old rats. The induction of hsp70 synthesis was significantly lower (37%) for hepatocytes isolated from old rats compared to hepatocytes isolated from young adult rats. However, neither the basal level nor the induced level of hsc70 synthesis changed significantly with age. Thus, aging resulted in a decrease in the ability of hepatocytes to synthesize hsp70 in response to hyperthermia; this effect, however, was specific for hsp70.

Original languageEnglish (US)
JournalJournals of Gerontology
Volume48
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

HSP70 Heat-Shock Proteins
Hepatocytes
Hot Temperature
Young Adult
Shock
Proteins
Fever
Induced Hyperthermia
Temperature
Inbred F344 Rats
Methionine
Cell Survival
Molecular Weight
Monoclonal Antibodies
Genes

ASJC Scopus subject areas

  • Aging

Cite this

Wu, B., Gu, M. J., Heydari, A. R., & Richardson, A. (1993). The effect of age on the synthesis of two heat shock proteins in the HSP70 family. Journals of Gerontology, 48(2).

The effect of age on the synthesis of two heat shock proteins in the HSP70 family. / Wu, B.; Gu, M. J.; Heydari, A. R.; Richardson, A.

In: Journals of Gerontology, Vol. 48, No. 2, 1993.

Research output: Contribution to journalArticle

Wu, B, Gu, MJ, Heydari, AR & Richardson, A 1993, 'The effect of age on the synthesis of two heat shock proteins in the HSP70 family', Journals of Gerontology, vol. 48, no. 2.
Wu, B. ; Gu, M. J. ; Heydari, A. R. ; Richardson, A. / The effect of age on the synthesis of two heat shock proteins in the HSP70 family. In: Journals of Gerontology. 1993 ; Vol. 48, No. 2.
@article{3db93568bfc749099dc71eab36bc276c,
title = "The effect of age on the synthesis of two heat shock proteins in the HSP70 family",
abstract = "Hepatocytes isolated from male F344 rats were exposed to elevated temperatures (40 °C to 45 °C), and the incorporation of [35S]-L- methionine into proteins was measured from fluorograms of two-dimensional polyacrylamide gels. The synthesis of two proteins was induced by temperatures of 40 °C to 42.5 °C; however, 45 °C inhibited the synthesis of all proteins. Based on their apparent molecular weights and pI values and their recognition by a monoclonal antibody to the HSP70 gene family, the two proteins induced by hyperthermia were found to be the highly heat-inducible hsp70 and the constitutive hsc70. Because a heat shock of 42.5 °C for 30 minutes had very little effect on cell viability and induced the synthesis of hsp70 and hsc70, this heat shock was used to study the effect of age on the ability of hepatocytes to respond to the stress of hyperthermia. Neither hepatocytes isolated from young adult (5-7 months) nor old (25-27 months) rats synthesized detectable amounts of hsp70 when incubated at 37 °C. However, heat shock induced the synthesis of both hsp70 and hsc70 in hepatocytes isolated from young adult and old rats. The induction of hsp70 synthesis was significantly lower (37{\%}) for hepatocytes isolated from old rats compared to hepatocytes isolated from young adult rats. However, neither the basal level nor the induced level of hsc70 synthesis changed significantly with age. Thus, aging resulted in a decrease in the ability of hepatocytes to synthesize hsp70 in response to hyperthermia; this effect, however, was specific for hsp70.",
author = "B. Wu and Gu, {M. J.} and Heydari, {A. R.} and A. Richardson",
year = "1993",
language = "English (US)",
volume = "48",
journal = "Journal of Gerontology: Social Sciences",
issn = "0022-1422",
publisher = "Gerontological Society of America",
number = "2",

}

TY - JOUR

T1 - The effect of age on the synthesis of two heat shock proteins in the HSP70 family

AU - Wu, B.

AU - Gu, M. J.

AU - Heydari, A. R.

AU - Richardson, A.

PY - 1993

Y1 - 1993

N2 - Hepatocytes isolated from male F344 rats were exposed to elevated temperatures (40 °C to 45 °C), and the incorporation of [35S]-L- methionine into proteins was measured from fluorograms of two-dimensional polyacrylamide gels. The synthesis of two proteins was induced by temperatures of 40 °C to 42.5 °C; however, 45 °C inhibited the synthesis of all proteins. Based on their apparent molecular weights and pI values and their recognition by a monoclonal antibody to the HSP70 gene family, the two proteins induced by hyperthermia were found to be the highly heat-inducible hsp70 and the constitutive hsc70. Because a heat shock of 42.5 °C for 30 minutes had very little effect on cell viability and induced the synthesis of hsp70 and hsc70, this heat shock was used to study the effect of age on the ability of hepatocytes to respond to the stress of hyperthermia. Neither hepatocytes isolated from young adult (5-7 months) nor old (25-27 months) rats synthesized detectable amounts of hsp70 when incubated at 37 °C. However, heat shock induced the synthesis of both hsp70 and hsc70 in hepatocytes isolated from young adult and old rats. The induction of hsp70 synthesis was significantly lower (37%) for hepatocytes isolated from old rats compared to hepatocytes isolated from young adult rats. However, neither the basal level nor the induced level of hsc70 synthesis changed significantly with age. Thus, aging resulted in a decrease in the ability of hepatocytes to synthesize hsp70 in response to hyperthermia; this effect, however, was specific for hsp70.

AB - Hepatocytes isolated from male F344 rats were exposed to elevated temperatures (40 °C to 45 °C), and the incorporation of [35S]-L- methionine into proteins was measured from fluorograms of two-dimensional polyacrylamide gels. The synthesis of two proteins was induced by temperatures of 40 °C to 42.5 °C; however, 45 °C inhibited the synthesis of all proteins. Based on their apparent molecular weights and pI values and their recognition by a monoclonal antibody to the HSP70 gene family, the two proteins induced by hyperthermia were found to be the highly heat-inducible hsp70 and the constitutive hsc70. Because a heat shock of 42.5 °C for 30 minutes had very little effect on cell viability and induced the synthesis of hsp70 and hsc70, this heat shock was used to study the effect of age on the ability of hepatocytes to respond to the stress of hyperthermia. Neither hepatocytes isolated from young adult (5-7 months) nor old (25-27 months) rats synthesized detectable amounts of hsp70 when incubated at 37 °C. However, heat shock induced the synthesis of both hsp70 and hsc70 in hepatocytes isolated from young adult and old rats. The induction of hsp70 synthesis was significantly lower (37%) for hepatocytes isolated from old rats compared to hepatocytes isolated from young adult rats. However, neither the basal level nor the induced level of hsc70 synthesis changed significantly with age. Thus, aging resulted in a decrease in the ability of hepatocytes to synthesize hsp70 in response to hyperthermia; this effect, however, was specific for hsp70.

UR - http://www.scopus.com/inward/record.url?scp=0027511556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027511556&partnerID=8YFLogxK

M3 - Article

C2 - 8473688

AN - SCOPUS:0027511556

VL - 48

JO - Journal of Gerontology: Social Sciences

JF - Journal of Gerontology: Social Sciences

SN - 0022-1422

IS - 2

ER -