The effect of age on protein synthesis and ribosome aggregation to messenger RNA in rat liver

Donald K. Layman, George A. Ricca, Arlan Richardson

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Abstract

The level of cell-free protein synthesis and ribosome aggregation to mRNA was determined in the livers of rats from 1 to 18 months of age. Protein synthesis by liver postmitochondrial supernatant fraction decreased rapidly in the first 9 months, with little change thereafter. The decrease in protein synthesis was associated with the ribosomal fraction of the postmitochondrial supernatant fraction. Ribosome aggregation to mRNA, as determined by sucrose density gradient centrifugation, decreased rapidly during the first 9 months and changed little thereafter. The decrease in protein synthesis with increasing age appears to be the result of reduced ribosome aggregation to mRNA. This decrease in aggregation was not due to changes in ribonuclease or the availability of amino acids for protein synthesis but appeared to be an age-related phenomenon.

Original languageEnglish (US)
Pages (from-to)246-254
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume173
Issue number1
DOIs
StatePublished - Mar 1976

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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