TY - JOUR
T1 - The effect of a low-fat, high fiber, fruit and vegetable intervention on rectal mucosal proliferation
AU - Pfeiffer, Ruth
AU - McShane, Lisa
AU - Wargovich, Michael
AU - Burt, Randall
AU - Kikendall, Walter
AU - Lawson, Michael
AU - Lanza, Elaine
AU - Schatzkin, Arthur
PY - 2003/9/15
Y1 - 2003/9/15
N2 - BACKGROUND. Because studies of diet and colorectal carcinoma tend to be large and complex, researchers have long been interested in the investigation of dietary exposures in relation to putative intermediate markers of large bowel malignancy, such as colorectal epithelial cell proliferation. The basic hypothesis underlying these investigations is that specific dietary components may reduce or increase the rate of cell proliferation, which, in turn, may reduce or increase neoplastic changes in the large bowel. METHODS. The authors assessed the effects of a 4-year, low-fat, high-fiber, fruit and vegetable - enriched dietary intervention on colorectal epithelial cell proliferation among 399 participants from the Polyp Prevention Trial, a randomized multicenter trial with adenoma recurrence as the primary endpoint. Rectal biopsies were taken from flat, normal appearing mucosa on patients at baseline, after 1 year, and after 4 years. Two assays, bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA), were used to evaluate two summary measures of proliferation: the labeling index (LI) and the proliferative height (PH). RESULTS. There were no significant differences between changes in LI and PH over the 4-year period for the intervention and control groups. This finding parallels the finding in the larger primary study, in which the dietary intervention did not alter adenoma recurrence rates. CONCLUSIONS. A low-fat, high-fiber, fruit and vegetable-enriched dietary intervention did not alter rectal mucosal cell proliferation rates.
AB - BACKGROUND. Because studies of diet and colorectal carcinoma tend to be large and complex, researchers have long been interested in the investigation of dietary exposures in relation to putative intermediate markers of large bowel malignancy, such as colorectal epithelial cell proliferation. The basic hypothesis underlying these investigations is that specific dietary components may reduce or increase the rate of cell proliferation, which, in turn, may reduce or increase neoplastic changes in the large bowel. METHODS. The authors assessed the effects of a 4-year, low-fat, high-fiber, fruit and vegetable - enriched dietary intervention on colorectal epithelial cell proliferation among 399 participants from the Polyp Prevention Trial, a randomized multicenter trial with adenoma recurrence as the primary endpoint. Rectal biopsies were taken from flat, normal appearing mucosa on patients at baseline, after 1 year, and after 4 years. Two assays, bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA), were used to evaluate two summary measures of proliferation: the labeling index (LI) and the proliferative height (PH). RESULTS. There were no significant differences between changes in LI and PH over the 4-year period for the intervention and control groups. This finding parallels the finding in the larger primary study, in which the dietary intervention did not alter adenoma recurrence rates. CONCLUSIONS. A low-fat, high-fiber, fruit and vegetable-enriched dietary intervention did not alter rectal mucosal cell proliferation rates.
KW - Adenoma recurrence
KW - Dietary intervention
KW - Intermediate endpoint
KW - Polyp Prevention Trial
KW - Rectal mucosal cell proliferation
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U2 - 10.1002/cncr.11608
DO - 10.1002/cncr.11608
M3 - Article
C2 - 12973839
AN - SCOPUS:0041917138
VL - 98
SP - 1161
EP - 1168
JO - Cancer
JF - Cancer
SN - 0008-543X
IS - 6
ER -