The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

Hyun Suk Kim; Qiujia Chen; Sung Kyung Kim; Jac A. Nickoloff; Robert Hromas; Millie M. Georgiadis; Suk Hee Lee

doi:10.1074/jbc.M113.533216

The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

Hyun Suk Kim, Qiujia Chen, Sung Kyung Kim, Jac A. Nickoloff, Robert Hromas, Millie M. Georgiadis, Suk Hee Lee

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.

Original language	English (US)
Pages (from-to)	10930-10938
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	289
Issue number	15
DOIs	https://doi.org/10.1074/jbc.M113.533216
State	Published - Apr 11 2014
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biochemistry
Cell Biology

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title = "The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart",

abstract = "Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.",

author = "Kim, {Hyun Suk} and Qiujia Chen and Kim, {Sung Kyung} and Nickoloff, {Jac A.} and Robert Hromas and Georgiadis, {Millie M.} and Lee, {Suk Hee}",

year = "2014",

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doi = "10.1074/jbc.M113.533216",

language = "English (US)",

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T1 - The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

AU - Kim, Hyun Suk

AU - Chen, Qiujia

AU - Kim, Sung Kyung

AU - Nickoloff, Jac A.

AU - Hromas, Robert

AU - Georgiadis, Millie M.

AU - Lee, Suk Hee

PY - 2014/4/11

Y1 - 2014/4/11

N2 - Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.

AB - Background: Metnase, a transposase-containing DNA repair protein, retains DNA cleavage activity with a DDN motif. Results: Substitution with the ancestral transposase DDD/DDE catalytic motif results in a decrease in ssDNA binding and ss-overhang cleavage activities. Conclusion: The DDN motif is required for Metnase DNA repair activities. Significance: Understanding the requirements for catalytic activity provides insights on how Metnase functions as a DNA repair protein.

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JO - Journal of Biological Chemistry

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The DDN catalytic motif is required for Metnase functions in non-homologous end joining (NHEJ) repair and replication restart

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