The complete sequence of marine bacteriophage VpV262 infecting Vibrio parahaemolyticus indicates that an ancestral component of a T7 viral supergroup is widespread in the marine environment

Stephen C. Hardies, André M. Comeau, Philip Serwer, Curtis A. Suttle

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

The 46,012-bp sequence of the marine bacteriophage VpV262 infecting the bacterium Vibrio parahaemolyticus is reported. The VpV262 sequence reveals that it is a distant relative of marine Roscophage SIO1, and an even more distant relative of coliphage T7. VpV262 and SIO1 appear to represent a widespread marine phage group that lacks an RNA polymerase gene and is ancestral to the T7-like phages. We propose that this group together with the T7-like phages be designated as the T7 supergroup. The ancestral head structure gene module for the T7 supcrgroup was reconstructed by using sensitive biased Psi-blast searches supplemented by statistical support derived from gene order. In the early and replicative segments, these phages have participated in extensive interchange with the viral gene pool. VpV262 carries a different replicativc module than SIO1 and the T7-like phages.

Original languageEnglish (US)
Pages (from-to)359-371
Number of pages13
JournalVirology
Volume310
Issue number2
DOIs
Publication statusPublished - Jun 5 2003

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Keywords

  • Bacteriophage
  • Marine phage
  • Vibriophage

ASJC Scopus subject areas

  • Virology

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