The chemical mutagen dimethyl sulphate induces homologous recombination of plasmid DNA by increasing the binding of RecA protein to duplex DNA

Grigory L. Dianov, Murat K. Saparbaev, Alexander V. Mazin, Rudolf I. Salganik

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The role of different DNA damages in the stimulation of homologous recombination was studied by using an in vivo plasmid recombination assay. Dimethyl sulphate (DMS) treatment of plasmid DNA induced a 20-50-fold increase in the frequency of recombinational events. DMS treatment also stimulated RecA protein binding to double-stranded DNA. In contrast, plasmid DNA containing uracil, which, like DMS, is also subject to repair, was less effective in stimulation of recombination. The ability of purified RecA protein to bind DMS-treated or uracil-containing DNA was tested by measuring its ATPase activity. The result indicates that DMS treatment, but not uracil incorportion, stimulates RecA protein binding to DNA. We conclude, that the main reason (or the first step) for stimulation of recombination by mutagens is activation of RecA binding to damaged DNA.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume249
Issue number1
DOIs
StatePublished - Jul 1991
Externally publishedYes

Keywords

  • Dimethyl sulphate
  • mutagen-stimulated
  • Plasmid recombination
  • RecA binding
  • Uracil

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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