The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis

Angel Viudes; Anna Lazzell; Sofia Perea; William R. Kirkpatrick; Javier Peman; Thomas F. Patterson; Jose P. Martinez; José L. López-Ribot

doi:10.1016/S0378-1097(04)00042-4

The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis

Angel Viudes, Anna Lazzell, Sofia Perea, William R. Kirkpatrick, Javier Peman, Thomas F. Patterson, Jose P. Martinez, José L. López-Ribot

Division of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The 58-kDa surface mannoprotein of Candida albicans (mp58) elicits strong antibody responses during infection. Epitope mapping with sera from patients with candidiasis and control individuals indicated the presence of multiple IgG-reactive continuous epitopes on the protein, expanding both the amino- and carboxy-terminal domains and several internal regions. These immunoreactive regions were similar to the ones previously identified using sera from immunized animals. Two of the epitopic regions (including the C-terminal domain) showed increased reactivity with antibodies present in sera from patients with candidiasis as compared to control individuals. Patients who survived the infection displayed increased antibody reactivity towards the C-terminal epitope as compared to those succumbing to candidiasis. A monoclonal antibody directed towards this epitopic region conferred protection in serum therapy experiments in a murine model of hematogenously disseminated candidiasis. Together, these observations indicate the carboxy-terminal antibody binding domain of C. albicans mp58 represents a protective epitope during candidiasis.

Original language	English (US)
Pages (from-to)	133-138
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	232
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(04)00042-4
State	Published - Mar 19 2004

Keywords

Candida albicans
Monoclonal antibody
Protective epitope

ASJC Scopus subject areas

General Medicine

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10.1016/S0378-1097(04)00042-4

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@article{18b76f12f87843d094fdda4763819218,

title = "The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis",

abstract = "The 58-kDa surface mannoprotein of Candida albicans (mp58) elicits strong antibody responses during infection. Epitope mapping with sera from patients with candidiasis and control individuals indicated the presence of multiple IgG-reactive continuous epitopes on the protein, expanding both the amino- and carboxy-terminal domains and several internal regions. These immunoreactive regions were similar to the ones previously identified using sera from immunized animals. Two of the epitopic regions (including the C-terminal domain) showed increased reactivity with antibodies present in sera from patients with candidiasis as compared to control individuals. Patients who survived the infection displayed increased antibody reactivity towards the C-terminal epitope as compared to those succumbing to candidiasis. A monoclonal antibody directed towards this epitopic region conferred protection in serum therapy experiments in a murine model of hematogenously disseminated candidiasis. Together, these observations indicate the carboxy-terminal antibody binding domain of C. albicans mp58 represents a protective epitope during candidiasis.",

keywords = "Candida albicans, Monoclonal antibody, Protective epitope",

author = "Angel Viudes and Anna Lazzell and Sofia Perea and Kirkpatrick, {William R.} and Javier Peman and Patterson, {Thomas F.} and Martinez, {Jose P.} and L{\'o}pez-Ribot, {Jos{\'e} L.}",

note = "Funding Information: This work was supported by Public Health Service Grant 1 R29 AI42401 (to J.L.L.-R.). J.L.L.-R. is the recipient of a New Investigator Award in Molecular Pathogenic Mycology from the Burroughs Wellcome Fund. A.V. was the recipient of postdoctoral fellowships from the Sociedad Espa{\~n}ola de Quimioterapia (SEQ) and Sociedad Espa{\~n}ola de Enfermedades Infecciosas y Microbiolog{\'ı}a Cl{\'ı}nica (SEIMC-Fundaci{\'o}n Wellcome). S.P. acknowledges the receipt of a NATO postdoctoral fellowship. Synthesis of the C-terminal synthetic decapeptide was performed at the Protein Core Facility at UTHSCSA. Additional support was provided by Pfizer Inc. and Inhibitex Inc.",

year = "2004",

month = mar,

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doi = "10.1016/S0378-1097(04)00042-4",

language = "English (US)",

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pages = "133--138",

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T1 - The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis

AU - Viudes, Angel

AU - Lazzell, Anna

AU - Perea, Sofia

AU - Kirkpatrick, William R.

AU - Peman, Javier

AU - Patterson, Thomas F.

AU - Martinez, Jose P.

AU - López-Ribot, José L.

N1 - Funding Information: This work was supported by Public Health Service Grant 1 R29 AI42401 (to J.L.L.-R.). J.L.L.-R. is the recipient of a New Investigator Award in Molecular Pathogenic Mycology from the Burroughs Wellcome Fund. A.V. was the recipient of postdoctoral fellowships from the Sociedad Española de Quimioterapia (SEQ) and Sociedad Española de Enfermedades Infecciosas y Microbiologı́a Clı́nica (SEIMC-Fundación Wellcome). S.P. acknowledges the receipt of a NATO postdoctoral fellowship. Synthesis of the C-terminal synthetic decapeptide was performed at the Protein Core Facility at UTHSCSA. Additional support was provided by Pfizer Inc. and Inhibitex Inc.

PY - 2004/3/19

Y1 - 2004/3/19

N2 - The 58-kDa surface mannoprotein of Candida albicans (mp58) elicits strong antibody responses during infection. Epitope mapping with sera from patients with candidiasis and control individuals indicated the presence of multiple IgG-reactive continuous epitopes on the protein, expanding both the amino- and carboxy-terminal domains and several internal regions. These immunoreactive regions were similar to the ones previously identified using sera from immunized animals. Two of the epitopic regions (including the C-terminal domain) showed increased reactivity with antibodies present in sera from patients with candidiasis as compared to control individuals. Patients who survived the infection displayed increased antibody reactivity towards the C-terminal epitope as compared to those succumbing to candidiasis. A monoclonal antibody directed towards this epitopic region conferred protection in serum therapy experiments in a murine model of hematogenously disseminated candidiasis. Together, these observations indicate the carboxy-terminal antibody binding domain of C. albicans mp58 represents a protective epitope during candidiasis.

AB - The 58-kDa surface mannoprotein of Candida albicans (mp58) elicits strong antibody responses during infection. Epitope mapping with sera from patients with candidiasis and control individuals indicated the presence of multiple IgG-reactive continuous epitopes on the protein, expanding both the amino- and carboxy-terminal domains and several internal regions. These immunoreactive regions were similar to the ones previously identified using sera from immunized animals. Two of the epitopic regions (including the C-terminal domain) showed increased reactivity with antibodies present in sera from patients with candidiasis as compared to control individuals. Patients who survived the infection displayed increased antibody reactivity towards the C-terminal epitope as compared to those succumbing to candidiasis. A monoclonal antibody directed towards this epitopic region conferred protection in serum therapy experiments in a murine model of hematogenously disseminated candidiasis. Together, these observations indicate the carboxy-terminal antibody binding domain of C. albicans mp58 represents a protective epitope during candidiasis.

KW - Candida albicans

KW - Monoclonal antibody

KW - Protective epitope

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JO - FEMS Microbiology Letters

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The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis

Abstract

Keywords

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