The C-terminal antibody binding domain of Candida albicans mp58 represents a protective epitope during candidiasis

Angel Viudes, Anna Lazzell, Sofia Perea, William R. Kirkpatrick, Javier Peman, Thomas F. Patterson, Jose P. Martinez, José L. López-Ribot

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The 58-kDa surface mannoprotein of Candida albicans (mp58) elicits strong antibody responses during infection. Epitope mapping with sera from patients with candidiasis and control individuals indicated the presence of multiple IgG-reactive continuous epitopes on the protein, expanding both the amino- and carboxy-terminal domains and several internal regions. These immunoreactive regions were similar to the ones previously identified using sera from immunized animals. Two of the epitopic regions (including the C-terminal domain) showed increased reactivity with antibodies present in sera from patients with candidiasis as compared to control individuals. Patients who survived the infection displayed increased antibody reactivity towards the C-terminal epitope as compared to those succumbing to candidiasis. A monoclonal antibody directed towards this epitopic region conferred protection in serum therapy experiments in a murine model of hematogenously disseminated candidiasis. Together, these observations indicate the carboxy-terminal antibody binding domain of C. albicans mp58 represents a protective epitope during candidiasis.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
JournalFEMS Microbiology Letters
Volume232
Issue number2
DOIs
StatePublished - Mar 19 2004

Keywords

  • Candida albicans
  • Monoclonal antibody
  • Protective epitope

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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