The baboon gene for apolipoprotein A-I: characterization ofacDNA clone and identification of DNA polymorphisms for genetic studies of cholesterol metabolism

James E. Hixson, Shifra Borenstein, Laura A. Cox, David L. Rainwater, John L. VandeBerg

    Research output: Contribution to journalArticlepeer-review

    21 Scopus citations

    Abstract

    We have isolated and sequenced a baboon apolipoprotein A-I (ApoA-I) clone from a liver cDNA library using a human cDNA hybridization probe. This baboon cDNA contains the entire ApoA-I coding region (801 bp, 267 aa), a 3' untranslated region, and a poly(A) tail. Among comparisons with apoAI sequences from other species, the baboon cDNA is most similar to that of the cynomolgus macaque (99.2% homologous) and least similar to the rat sequence (72.6% homologous). A high frequency of nonsynonymous substitutions are observed by alignment of baboon and human apoAI cDNAs, but comparisons ofhydrophilicity profiles show that protein structure is conserved by substitutions of aa with similar properties. A polymorphic PstI cleavage site was identified by Southern blot analysis and subsequently mapped to the 5' end of the baboon apoAI gene. To identify effects of apoAI allelic variation on cholesterol metabolism, we used immunoblotting to compare the distributions of ApoA-I among lipoprotein size classes in baboons from each genotype under basal and atherogenic diets. We observed an increase of ApoA-I in high density lipoprotein (size class 1) particles after atherogenic diets in homozygotes for one allele, as compared to slight decreases in the other genotypes.

    Original languageEnglish (US)
    Pages (from-to)483-490
    Number of pages8
    JournalGene
    Volume74
    Issue number2
    DOIs
    StatePublished - Dec 30 1988

    Keywords

    • Recombinant DNA
    • cDNA library
    • gene markers
    • high density lipoproteins
    • liver
    • primate model

    ASJC Scopus subject areas

    • Genetics

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