The association of biomolecular resource facilities proteomics research group 2006 study: Relative protein quantitation

Cristoph W. Turck, Arnold M. Falick, Jeffrey A. Kowalak, William S. Lane, Kathryn S. Lilley, Brett S. Phinney, Susan T. Weintraub, H. Ewa Witkowska, Nathan A. Yates

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

The determination of differences in relative protein abundance is a critical aspect of proteomics research that is increasingly used to answer diverse biological questions. The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 study was a quantitative proteomics project in which the aim was to determine the identity and the relative amounts of eight proteins in two mixtures. There are numerous methodologies available to study the relative abundance of proteins between samples, but to date, there are few examples of studies that have compared these different approaches. For the 2006 Proteomics Research Group study, there were 52 paticipants who used a wide variety of gel electrophoresis-, HPLC-, and mass spectrometry-based methods for relative quantitation. The quantitative data arising from this study were evaluated along with several other experimental details relevant to the methodologies used.

Original languageEnglish (US)
Pages (from-to)1291-1298
Number of pages8
JournalMolecular and Cellular Proteomics
Volume6
Issue number8
DOIs
StatePublished - Aug 2007

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

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