The aromatic residue content of the enzyme rhodanese

Richard D. Baillie, Paul M. Horowitz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The aromatic amino acid composition of the enzyme rhodanese has been redetermined. Previous reports have varied from 5 to 11 tryptophans per 26 alanine residues. The present work has quantitated the aromatic residues by a combination of amino acid analysis, solvent perturbation difference spectroscopy, specific residue modification and direct ultraviolet spectral analysis. These methods indicate that rhodanese contains 10 tyrosimes, eight tryptophans and 16 phenylalanines per 26 alanine residues. The result for tyrosine and phenylalanine are in reasonable agreement with previous results.

Original languageEnglish (US)
Pages (from-to)594-599
Number of pages6
JournalBBA - Protein Structure
Volume427
Issue number2
DOIs
StatePublished - Apr 14 1976
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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