TY - JOUR
T1 - The aromatic residue content of the enzyme rhodanese
AU - Baillie, Richard D.
AU - Horowitz, Paul M.
N1 - Funding Information:
Acknowledgment is made to the donors of The Petroleum Research Fund, administered by the American Chemical Society, for the support of this research.
PY - 1976/4/14
Y1 - 1976/4/14
N2 - The aromatic amino acid composition of the enzyme rhodanese has been redetermined. Previous reports have varied from 5 to 11 tryptophans per 26 alanine residues. The present work has quantitated the aromatic residues by a combination of amino acid analysis, solvent perturbation difference spectroscopy, specific residue modification and direct ultraviolet spectral analysis. These methods indicate that rhodanese contains 10 tyrosimes, eight tryptophans and 16 phenylalanines per 26 alanine residues. The result for tyrosine and phenylalanine are in reasonable agreement with previous results.
AB - The aromatic amino acid composition of the enzyme rhodanese has been redetermined. Previous reports have varied from 5 to 11 tryptophans per 26 alanine residues. The present work has quantitated the aromatic residues by a combination of amino acid analysis, solvent perturbation difference spectroscopy, specific residue modification and direct ultraviolet spectral analysis. These methods indicate that rhodanese contains 10 tyrosimes, eight tryptophans and 16 phenylalanines per 26 alanine residues. The result for tyrosine and phenylalanine are in reasonable agreement with previous results.
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U2 - 10.1016/0005-2795(76)90202-6
DO - 10.1016/0005-2795(76)90202-6
M3 - Article
C2 - 1268220
AN - SCOPUS:0017254966
VL - 427
SP - 594
EP - 599
JO - BBA - Protein Structure
JF - BBA - Protein Structure
SN - 1570-9639
IS - 2
ER -