The age-related decline in interleukin-3 expression in mice

Li Ding-Dong Li, Chien Yu-Kun Chien, Gu Mao-Zhi, Arlan Richardson, H. Tak Cheung

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.

Original languageEnglish (US)
Pages (from-to)1215-1222
Number of pages8
JournalLife Sciences
Volume43
Issue number15
DOIs
StatePublished - 1988
Externally publishedYes

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Interleukin-3
Lymphocytes
Messenger RNA
Concanavalin A
Interleukins
Inbred C57BL Mouse
Northern Blotting
Interleukin-2
Spleen

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ding-Dong Li, L., Yu-Kun Chien, C., Mao-Zhi, G., Richardson, A., & Tak Cheung, H. (1988). The age-related decline in interleukin-3 expression in mice. Life Sciences, 43(15), 1215-1222. https://doi.org/10.1016/0024-3205(88)90211-1

The age-related decline in interleukin-3 expression in mice. / Ding-Dong Li, Li; Yu-Kun Chien, Chien; Mao-Zhi, Gu; Richardson, Arlan; Tak Cheung, H.

In: Life Sciences, Vol. 43, No. 15, 1988, p. 1215-1222.

Research output: Contribution to journalArticle

Ding-Dong Li, L, Yu-Kun Chien, C, Mao-Zhi, G, Richardson, A & Tak Cheung, H 1988, 'The age-related decline in interleukin-3 expression in mice', Life Sciences, vol. 43, no. 15, pp. 1215-1222. https://doi.org/10.1016/0024-3205(88)90211-1
Ding-Dong Li L, Yu-Kun Chien C, Mao-Zhi G, Richardson A, Tak Cheung H. The age-related decline in interleukin-3 expression in mice. Life Sciences. 1988;43(15):1215-1222. https://doi.org/10.1016/0024-3205(88)90211-1
Ding-Dong Li, Li ; Yu-Kun Chien, Chien ; Mao-Zhi, Gu ; Richardson, Arlan ; Tak Cheung, H. / The age-related decline in interleukin-3 expression in mice. In: Life Sciences. 1988 ; Vol. 43, No. 15. pp. 1215-1222.
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