The age-related decline in interleukin-3 expression in mice

Li Ding-Dong Li; Chien Yu-Kun Chien; Gu Mao-Zhi; Arlan Richardson; H. Tak Cheung

doi:10.1016/0024-3205(88)90211-1

The age-related decline in interleukin-3 expression in mice

Li Ding-Dong Li, Chien Yu-Kun Chien, Gu Mao-Zhi, Arlan Richardson, H. Tak Cheung

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.

Original language	English (US)
Pages (from-to)	1215-1222
Number of pages	8
Journal	Life Sciences
Volume	43
Issue number	15
DOIs	https://doi.org/10.1016/0024-3205(88)90211-1
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(88)90211-1

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title = "The age-related decline in interleukin-3 expression in mice",

abstract = "Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.",

author = "{Ding-Dong Li}, Li and {Yu-Kun Chien}, Chien and Gu Mao-Zhi and Arlan Richardson and {Tak Cheung}, H.",

note = "Funding Information: ACKNOWLEDGMENT This work was supported by grants AG 04520, AG 01548, and AI 20343 from National Institutes of Health, Bethesda, M~. The authors thank the following investigators for generously supplying the cell lines and eDNA probes: FDC-P1 cells from Dr. Sandra K. Ruscetti (National Cancer Institute, Bethesda, MD), C3%L-20 cells from Dr. Charles Orosz (Therapeutic Immunology Laboratories, Ohio State University, Columbus, OH), mouse IL-2 eDNA probe from Dr. Tony Burgess, (Institute for Cancer Research, Melbourne, Australia), and the IL-3 eDNA probe from Dr. K. Arai (DNAX Research Institute, Palo Alto, CA).",

year = "1988",

doi = "10.1016/0024-3205(88)90211-1",

language = "English (US)",

volume = "43",

pages = "1215--1222",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

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TY - JOUR

T1 - The age-related decline in interleukin-3 expression in mice

AU - Ding-Dong Li, Li

AU - Yu-Kun Chien, Chien

AU - Mao-Zhi, Gu

AU - Richardson, Arlan

AU - Tak Cheung, H.

N1 - Funding Information: ACKNOWLEDGMENT This work was supported by grants AG 04520, AG 01548, and AI 20343 from National Institutes of Health, Bethesda, M~. The authors thank the following investigators for generously supplying the cell lines and eDNA probes: FDC-P1 cells from Dr. Sandra K. Ruscetti (National Cancer Institute, Bethesda, MD), C3%L-20 cells from Dr. Charles Orosz (Therapeutic Immunology Laboratories, Ohio State University, Columbus, OH), mouse IL-2 eDNA probe from Dr. Tony Burgess, (Institute for Cancer Research, Melbourne, Australia), and the IL-3 eDNA probe from Dr. K. Arai (DNAX Research Institute, Palo Alto, CA).

PY - 1988

Y1 - 1988

N2 - Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.

AB - Spleen lymphocytes form 5- to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.

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The age-related decline in interleukin-3 expression in mice

Abstract

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