The -56T HLA-G promoter polymorphism is not associated with pre-eclampsia/eclampsia in Australian and New Zealand women

Vicki Doherty; Ashley Rush; Shaun Brennecke; Eric Moses

doi:10.1080/10641950500543780

The -56T HLA-G promoter polymorphism is not associated with pre-eclampsia/eclampsia in Australian and New Zealand women

Vicki Doherty, Ashley Rush, Shaun Brennecke, Eric Moses

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Objective: Decreased HLA-G expression has been linked to a number of pregnancy disorders, including preeclampsia, and a genetic basis for HLA-G regulation has yet to be found. The aim of this study was to determine whether a C-to-T base substitution 56 base pairs (bp) upstream from the HLA-G transcription start site is associated with preeclampsia. Methods: 277 nulliparous women consisting of 113 normotensive, 118 preeclamptic, and 46 eclamptic patients were typed for the -56T polymorphism using restriction fragment length polymorphism and allelic discrimination analysis. Results: -56T allele frequencies for eclamptic, preeclamptic, and normotensive women were 0.053, 0.030, and 0.035, respectively. A χ 2 test indicated that there was no significant association with the polymorphism in preeclamptic or eclamptic women with p > 0.05. Conclusion: The -56T HLA-G polymorphism is not associated with preeclampsia or eclampsia in our population.

Original language	English (US)
Pages (from-to)	63-71
Number of pages	9
Journal	Hypertension in Pregnancy
Volume	25
Issue number	2
DOIs	https://doi.org/10.1080/10641950500543780
State	Published - Jul 1 2006

Keywords

Allelic discrimination
HLA-G
Pre-eclampsia
Promoter polymorphism

ASJC Scopus subject areas

Internal Medicine
Obstetrics and Gynecology

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title = "The -56T HLA-G promoter polymorphism is not associated with pre-eclampsia/eclampsia in Australian and New Zealand women",

abstract = "Objective: Decreased HLA-G expression has been linked to a number of pregnancy disorders, including preeclampsia, and a genetic basis for HLA-G regulation has yet to be found. The aim of this study was to determine whether a C-to-T base substitution 56 base pairs (bp) upstream from the HLA-G transcription start site is associated with preeclampsia. Methods: 277 nulliparous women consisting of 113 normotensive, 118 preeclamptic, and 46 eclamptic patients were typed for the -56T polymorphism using restriction fragment length polymorphism and allelic discrimination analysis. Results: -56T allele frequencies for eclamptic, preeclamptic, and normotensive women were 0.053, 0.030, and 0.035, respectively. A χ 2 test indicated that there was no significant association with the polymorphism in preeclamptic or eclamptic women with p > 0.05. Conclusion: The -56T HLA-G polymorphism is not associated with preeclampsia or eclampsia in our population.",

keywords = "Allelic discrimination, HLA-G, Pre-eclampsia, Promoter polymorphism",

author = "Vicki Doherty and Ashley Rush and Shaun Brennecke and Eric Moses",

note = "Funding Information: The authors gratefully acknowledge the assistance of clinical research midwives Tracey Nielsen, Moira Stewart, and Sandy Walker in patient ascertainment, recruitment, and sampling and all the women and their families who participated in this study. This work was supported by Australian National Health and Medical Research Council project grant 970589, the University of Melbourne, the Royal Women{\textquoteright}s Hospital Melbourne, and Wake Forest University, Winston-Salem, NC, USA.",

year = "2006",

month = jul,

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doi = "10.1080/10641950500543780",

language = "English (US)",

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AU - Doherty, Vicki

AU - Rush, Ashley

AU - Brennecke, Shaun

AU - Moses, Eric

N1 - Funding Information: The authors gratefully acknowledge the assistance of clinical research midwives Tracey Nielsen, Moira Stewart, and Sandy Walker in patient ascertainment, recruitment, and sampling and all the women and their families who participated in this study. This work was supported by Australian National Health and Medical Research Council project grant 970589, the University of Melbourne, the Royal Women’s Hospital Melbourne, and Wake Forest University, Winston-Salem, NC, USA.

PY - 2006/7/1

Y1 - 2006/7/1

N2 - Objective: Decreased HLA-G expression has been linked to a number of pregnancy disorders, including preeclampsia, and a genetic basis for HLA-G regulation has yet to be found. The aim of this study was to determine whether a C-to-T base substitution 56 base pairs (bp) upstream from the HLA-G transcription start site is associated with preeclampsia. Methods: 277 nulliparous women consisting of 113 normotensive, 118 preeclamptic, and 46 eclamptic patients were typed for the -56T polymorphism using restriction fragment length polymorphism and allelic discrimination analysis. Results: -56T allele frequencies for eclamptic, preeclamptic, and normotensive women were 0.053, 0.030, and 0.035, respectively. A χ 2 test indicated that there was no significant association with the polymorphism in preeclamptic or eclamptic women with p > 0.05. Conclusion: The -56T HLA-G polymorphism is not associated with preeclampsia or eclampsia in our population.

AB - Objective: Decreased HLA-G expression has been linked to a number of pregnancy disorders, including preeclampsia, and a genetic basis for HLA-G regulation has yet to be found. The aim of this study was to determine whether a C-to-T base substitution 56 base pairs (bp) upstream from the HLA-G transcription start site is associated with preeclampsia. Methods: 277 nulliparous women consisting of 113 normotensive, 118 preeclamptic, and 46 eclamptic patients were typed for the -56T polymorphism using restriction fragment length polymorphism and allelic discrimination analysis. Results: -56T allele frequencies for eclamptic, preeclamptic, and normotensive women were 0.053, 0.030, and 0.035, respectively. A χ 2 test indicated that there was no significant association with the polymorphism in preeclamptic or eclamptic women with p > 0.05. Conclusion: The -56T HLA-G polymorphism is not associated with preeclampsia or eclampsia in our population.

KW - Allelic discrimination

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KW - Promoter polymorphism

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