Oligonucleotides remaining in the 70s Escherichia coli ribosomal particles after varying degrees of digestion with ribonuclease T1 were phosphorylated with polynucleotide kinase in the presence of γ-labeled32P-ATP. The resulting radioactively labeled RNA molecules were further digested with pancreatic ribonuclease and analyzed by a two-dimensional finger-printing technique. The numbers of labeled oligonucleotides were proportional to the duration of T1 digestion; most of these oligonucleotides yielded *pAp and/or *pCp as their 5′-end groups upon alkaline hydrolysis.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 5 1973|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology