The 5′-termini of the oligonucleotides from ribonuclease T1 digested E. coli ribosomes

J. C. Lee, I. V. Quintanilla

Research output: Contribution to journalArticlepeer-review


Oligonucleotides remaining in the 70s Escherichia coli ribosomal particles after varying degrees of digestion with ribonuclease T1 were phosphorylated with polynucleotide kinase in the presence of γ-labeled32P-ATP. The resulting radioactively labeled RNA molecules were further digested with pancreatic ribonuclease and analyzed by a two-dimensional finger-printing technique. The numbers of labeled oligonucleotides were proportional to the duration of T1 digestion; most of these oligonucleotides yielded *pAp and/or *pCp as their 5′-end groups upon alkaline hydrolysis.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Mar 5 1973

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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