Abstract
Oligonucleotides remaining in the 70s Escherichia coli ribosomal particles after varying degrees of digestion with ribonuclease T1 were phosphorylated with polynucleotide kinase in the presence of γ-labeled32P-ATP. The resulting radioactively labeled RNA molecules were further digested with pancreatic ribonuclease and analyzed by a two-dimensional finger-printing technique. The numbers of labeled oligonucleotides were proportional to the duration of T1 digestion; most of these oligonucleotides yielded *pAp and/or *pCp as their 5′-end groups upon alkaline hydrolysis.
Original language | English (US) |
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Pages (from-to) | 14-19 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 51 |
Issue number | 1 |
DOIs | |
State | Published - Mar 5 1973 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology