The σ(E) subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments

H. C. Carlson, W. G. Haldenwang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Bacillus subtilis cells harvested 3.5 h after the onset of sporulation (t3.5) were fractionated into extracts enriched in either mother cell or forespore components and were analyzed immunologically for σ(E) and its precursor protein, P31. We determined by Western blot (immunoblot) analysis that equivalent amounts of P31 and σ(E) were present in both mother cell and forespore extracts. This result implies that, although σ(E) is not synthesized until a stage in development when the cell is partitioned into progenitor forespore and mother cell compartments, it probably directs the transcription of genes that are expressed in both of these structures.

Original languageEnglish (US)
Pages (from-to)2216-2218
Number of pages3
JournalJournal of bacteriology
Volume171
Issue number4
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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