Bacillus subtilis cells harvested 3.5 h after the onset of sporulation (t3.5) were fractionated into extracts enriched in either mother cell or forespore components and were analyzed immunologically for σ(E) and its precursor protein, P31. We determined by Western blot (immunoblot) analysis that equivalent amounts of P31 and σ(E) were present in both mother cell and forespore extracts. This result implies that, although σ(E) is not synthesized until a stage in development when the cell is partitioned into progenitor forespore and mother cell compartments, it probably directs the transcription of genes that are expressed in both of these structures.
ASJC Scopus subject areas
- Molecular Biology